1idm

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[[Image:1idm.jpg|left|200px]]
 
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==3-ISOPROPYLMALATE DEHYDROGENASE, LOOP-DELETED CHIMERA==
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The line below this paragraph, containing "STRUCTURE_1idm", creates the "Structure Box" on the page.
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<StructureSection load='1idm' size='340' side='right'caption='[[1idm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1idm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IDM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1idm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idm OCA], [https://pdbe.org/1idm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1idm RCSB], [https://www.ebi.ac.uk/pdbsum/1idm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1idm ProSAT]</span></td></tr>
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{{STRUCTURE_1idm| PDB=1idm | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEU3_THET8 LEU3_THET8] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01033]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/1idm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1idm ConSurf].
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<div style="clear:both"></div>
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'''3-ISOPROPYLMALATE DEHYDROGENASE, LOOP-DELETED CHIMERA'''
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==See Also==
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*[[Isopropylmalate dehydrogenase|Isopropylmalate dehydrogenase]]
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__TOC__
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==Overview==
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</StructureSection>
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A loop-deleted mutant form of 3-isopropylmalate dehydrogenase from Thermus thermophilus was constructed to investigate the relationship between the flexibility of the structure and the thermostability of the enzyme. The structure of the mutant enzyme was determined by X-ray crystallography and was found to be almost the same as that of the native enzyme with a reduced temperature factor. Although the mutant protein had lost the flexible loop, its function and thermostability had remained unchanged. This phenomenon can be explained by an internal reprieve tolerance mechanism.
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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==About this Structure==
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[[Category: Moriyama H]]
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1IDM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDM OCA].
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[[Category: Ohzeki M]]
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[[Category: Sakurai M]]
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==Reference==
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[[Category: Sato M]]
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Structure of a loop-deleted variant of 3-isopropylmalate dehydrogenase from Thermus thermophilus: an internal reprieve tolerance mechanism., Sakurai M, Ohzeki M, Miyazaki K, Moriyama H, Sato M, Tanaka N, Oshima T, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):124-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299733 15299733]
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[[Category: Tanaka N]]
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[[Category: 3-isopropylmalate dehydrogenase]]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
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[[Category: Moriyama, H.]]
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[[Category: Ohzeki, M.]]
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[[Category: Sakurai, M.]]
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[[Category: Sato, M.]]
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[[Category: Tanaka, N.]]
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[[Category: Chimera]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:52:59 2008''
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Current revision

3-ISOPROPYLMALATE DEHYDROGENASE, LOOP-DELETED CHIMERA

PDB ID 1idm

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