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| - | [[Image:1ie1.gif|left|200px]] | |
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| - | <!--
| + | ==NMR Solution Structure of an In Vitro Selected RNA which is Sequence Specifically Recognized by Hamster Nucleolin RBD12.== |
| - | The line below this paragraph, containing "STRUCTURE_1ie1", creates the "Structure Box" on the page.
| + | <StructureSection load='1ie1' size='340' side='right'caption='[[1ie1]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1ie1]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE1 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie1 OCA], [https://pdbe.org/1ie1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie1 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1ie1| PDB=1ie1 | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''NMR Solution Structure of an In Vitro Selected RNA which is Sequence Specifically Recognized by Hamster Nucleolin RBD12.'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Allain FH-T]] |
| - | ==Overview== | + | [[Category: Bouvet P]] |
| - | Nucleolin is an abundant nucleolar protein which is essential for ribosome biogenesis. The first two of its four tandem RNA-binding domains (RBD12) specifically recognize a stem-loop structure containing a conserved UCCCGA sequence in the loop called the nucleolin-recognition element (NRE). We have determined the structure of the consensus SELEX NRE (sNRE) by NMR spectroscopy. In both the free and bound RNA the top part of the stem forms a loop E (or S-turn) motif. In the absence of protein, the structure of the hairpin loop is not well defined due to conformational heterogeneity, and appears to be in equilibrium between two families of conformations. Titrations of RBD1, RBD2, and RBD12 with the sNRE show that specific binding requires RBD12. In complex with RBD12, the hairpin loop interacts specifically with the protein and adopts a well-defined structure which shares some of the features of the free form. The loop E motif also has specific interactions with the protein. Implications of these findings for the mechanism of recognition of RNA structures by modular proteins are discussed.
| + | [[Category: Dieckmann T]] |
| - | | + | [[Category: Feigon J]] |
| - | ==About this Structure==
| + | [[Category: Finger LD]] |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE1 OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Recognition of pre-formed and flexible elements of an RNA stem-loop by nucleolin., Bouvet P, Allain FH, Finger LD, Dieckmann T, Feigon J, J Mol Biol. 2001 Jun 8;309(3):763-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11397095 11397095]
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| - | [[Category: Allain, F H-T.]] | + | |
| - | [[Category: Bouvet, P.]] | + | |
| - | [[Category: Dieckmann, T.]] | + | |
| - | [[Category: Feigon, J.]] | + | |
| - | [[Category: Finger, L D.]] | + | |
| - | [[Category: Eucaryotic loop e motif a-form helix flexible loop]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:53:49 2008''
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