8gu0
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a fungal halogenase RadH== | |
| + | <StructureSection load='8gu0' size='340' side='right'caption='[[8gu0]], [[Resolution|resolution]] 2.42Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8gu0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Floropilus_chiversii Floropilus chiversii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GU0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gu0 OCA], [https://pdbe.org/8gu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gu0 RCSB], [https://www.ebi.ac.uk/pdbsum/8gu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gu0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RADH_FLOCH RADH_FLOCH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RadH is one of the flavin-dependent halogenases that has previously exhibited promising catalytic activity towards hydroxycoumarin, hydroxyisoquinoline, and phenolic derivatives. Here, we evaluated new functional homologs of RadH and expanded its specificities for the halogenation of non-tryptophan-derived, heterocyclic scaffolds. Our investigation revealed that RadH could effectively halogenate hydroxyquinoline and hydroxybenzothiophene. Assay optimization studies revealed the need to balance the various co-factor concentrations and where a GDHi co-factor recycling system most significantly improves the conversion and efficiency of the reaction. A crystal structure of RadH was also obtained with a resolution of 2.4 A, and docking studies were conducted to pinpoint the binding and catalytic sites for substrates. | ||
| - | + | Further Characterization of Fungal Halogenase RadH and Its Homologs.,Peh G, Gunawan GA, Tay T, Tiong E, Tan LL, Jiang S, Goh YL, Ye S, Wong J, Brown CJ, Zhao H, Ang EL, Wong FT, Lim YH Biomolecules. 2023 Jul 6;13(7):1081. doi: 10.3390/biom13071081. PMID:37509117<ref>PMID:37509117</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Brown | + | <div class="pdbe-citations 8gu0" style="background-color:#fffaf0;"></div> |
| - | [[Category: Jiang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Floropilus chiversii]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Brown CJ]] | ||
| + | [[Category: Jiang SM]] | ||
Current revision
Crystal structure of a fungal halogenase RadH
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