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1ig0

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[[Image:1ig0.gif|left|200px]]
 
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==Crystal Structure of yeast Thiamin Pyrophosphokinase==
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The line below this paragraph, containing "STRUCTURE_1ig0", creates the "Structure Box" on the page.
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<StructureSection load='1ig0' size='340' side='right'caption='[[1ig0]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ig0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IG0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VIB:3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM'>VIB</scene></td></tr>
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{{STRUCTURE_1ig0| PDB=1ig0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ig0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ig0 OCA], [https://pdbe.org/1ig0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ig0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ig0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ig0 ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of yeast Thiamin Pyrophosphokinase'''
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== Function ==
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[https://www.uniprot.org/uniprot/THI80_YEAST THI80_YEAST] Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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BACKGROUND: Thiamin pyrophosphokinase (TPK) catalyzes the transfer of a pyrophosphate group from ATP to vitamin B1 (thiamin) to form the coenzyme thiamin pyrophosphate (TPP). Thus, TPK is important for the formation of a coenzyme required for central metabolic functions. TPK has no sequence homologs in the PDB and functions by an unknown mechanism. The TPK structure has been determined as a significant step toward elucidating its catalytic action. RESULTS: The crystal structure of Saccharomyces cerevisiae TPK complexed with thiamin has been determined at 1.8 A resolution. TPK is a homodimer, and each subunit consists of two domains. One domain resembles a Rossman fold with four alpha helices on each side of a 6 strand parallel beta sheet. The other domain has one 4 strand and one 6 strand antiparallel beta sheet, which form a flattened sandwich structure containing a jelly-roll topology. The active site is located in a cleft at the dimer interface and is formed from residues from domains of both subunits. The TPK dimer contains two compound active sites at the subunit interface. CONCLUSIONS: The structure of TPK with one substrate bound identifies the location of the thiamin binding site and probable catalytic residues. The structure also suggests a likely binding site for ATP. These findings are further supported by TPK sequence homologies. Although possessing no significant sequence homology with other pyrophospokinases, thiamin pyrophosphokinase may operate by a mechanism of pyrophosphoryl transfer similar to those described for pyrophosphokinases functioning in nucleotide biosynthesis.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/1ig0_consurf.spt"</scriptWhenChecked>
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1IG0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG0 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The crystal structure of yeast thiamin pyrophosphokinase., Baker LJ, Dorocke JA, Harris RA, Timm DE, Structure. 2001 Jun;9(6):539-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11435118 11435118]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ig0 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Baker L-J]]
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[[Category: Thiamine diphosphokinase]]
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[[Category: Dorocke JA]]
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[[Category: Baker, L J.]]
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[[Category: Harris RA]]
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[[Category: Dorocke, J A.]]
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[[Category: Timm DE]]
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[[Category: Harris, R A.]]
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[[Category: Timm, D E.]]
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[[Category: Alpha-beta-alpha]]
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[[Category: Beta sandwich]]
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[[Category: Compound active site]]
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[[Category: Protein-substrate complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:57:34 2008''
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Current revision

Crystal Structure of yeast Thiamin Pyrophosphokinase

PDB ID 1ig0

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