1ig8

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[[Image:1ig8.gif|left|200px]]
 
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==Crystal Structure of Yeast Hexokinase PII with the correct amino acid sequence==
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The line below this paragraph, containing "STRUCTURE_1ig8", creates the "Structure Box" on the page.
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<StructureSection load='1ig8' size='340' side='right'caption='[[1ig8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ig8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The February 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''The Glycolytic Enzymes'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_2 10.2210/rcsb_pdb/mom_2004_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IG8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1ig8| PDB=1ig8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ig8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ig8 OCA], [https://pdbe.org/1ig8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ig8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ig8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ig8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HXKB_YEAST HXKB_YEAST] Main glucose phosphorylating enzyme. May play a regulatory role in both induction and repression of gene expression by glucose.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/1ig8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ig8 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Yeast Hexokinase PII with the correct amino acid sequence'''
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==See Also==
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*[[Hexokinase 3D structures|Hexokinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose 6-phosphate and ADP. Two yeast hexokinase isozymes are known, namely PI and PII. The crystal structure of yeast hexokinase PII from Saccharomyces cerevisiae without substrate or competitive inhibitor is determined and refined in a tetragonal crystal form at 2.2-A resolution. The folding of the peptide chain is very similar to that of Schistosoma mansoni and previous yeast hexokinase models despite only 30% sequence identity between them. Distinct differences in conformation are found that account for the absence of glucose in the binding site. Comparison of the current model with S. mansoni and yeast hexokinase PI structures both complexed with glucose shows in atomic detail the rigid body domain closure and specific loop movements as glucose binds. A hydrophobic channel formed by strictly conserved hydrophobic residues in the small domain of the hexokinase is identified. The channel's mouth is close to the active site and passes through the small domain to its surface. The possible role of the observed channel in proton transfer is discussed.
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
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==About this Structure==
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1IG8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 1IG8 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb50_1.html The Glycolytic Enzymes]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG8 OCA].
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==Reference==
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The high resolution crystal structure of yeast hexokinase PII with the correct primary sequence provides new insights into its mechanism of action., Kuser PR, Krauchenco S, Antunes OA, Polikarpov I, J Biol Chem. 2000 Jul 7;275(27):20814-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10749890 10749890]
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[[Category: Hexokinase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
 
[[Category: The Glycolytic Enzymes]]
[[Category: The Glycolytic Enzymes]]
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[[Category: Antunes, O A.]]
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[[Category: Antunes OA]]
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[[Category: Krauchenco, S.]]
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[[Category: Krauchenco S]]
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[[Category: Kuser, P R.]]
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[[Category: Kuser PR]]
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[[Category: Polikarpov, I.]]
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[[Category: Polikarpov I]]
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[[Category: Cleft]]
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[[Category: Mixed alpha beta]]
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[[Category: Two domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:57:59 2008''
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Current revision

Crystal Structure of Yeast Hexokinase PII with the correct amino acid sequence

PDB ID 1ig8

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