8ek9
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the class A carbapenemase CRH-1 in complex with avibactam at 1.4 Angstrom resolution== | |
| + | <StructureSection load='8ek9' size='340' side='right'caption='[[8ek9]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ek9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chromobacterium_haemolyticum Chromobacterium haemolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EK9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ek9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ek9 OCA], [https://pdbe.org/8ek9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ek9 RCSB], [https://www.ebi.ac.uk/pdbsum/8ek9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ek9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1W0D7S2_9NEIS A0A1W0D7S2_9NEIS] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | KPC-2 is one of the most relevant serine-carbapenemases among the carbapenem-resistant Enterobacterales. We previously isolated from the environmental species Chromobacterium haemolyticum a class A CRH-1 beta-lactamase displaying 69% amino acid sequence identity with KPC-2. The objective of this study was to analyze the kinetic behavior and crystallographic structure of this beta-lactamase. Our results showed that CRH-1 can hydrolyze penicillins, cephalosporins (except ceftazidime), and carbapenems with similar efficacy compared to KPC-2. Inhibition kinetics showed that CRH-1 is not well inhibited by clavulanic acid, in contrast to efficient inhibition by avibactam (AVI). The high-resolution crystal of the apoenzyme showed that CRH-1 has a similar folding compared to other class A beta-lactamases. The CRH-1/AVI complex showed that AVI adopts a chair conformation, stabilized by hydrogen bonds to Ser70, Ser237, Asn132, and Thr235. Our findings highlight the biochemical and structural similarities of CRH-1 and KPC-2 and the potential clinical impact of this carbapenemase in the event of recruitment by pathogenic bacterial species. | ||
| - | + | Biochemical and Structural Characterization of CRH-1, a Carbapenemase from Chromobacterium haemolyticum Related to KPC beta-Lactamases.,Brunetti F, Ghiglione B, Gudeta DD, Gutkind G, Guardabassi L, Klinke S, Power P Antimicrob Agents Chemother. 2023 Jul 18;67(7):e0006123. doi: , 10.1128/aac.00061-23. Epub 2023 Jun 5. PMID:37272821<ref>PMID:37272821</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8ek9" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Ghiglione | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Power | + | [[Category: Chromobacterium haemolyticum]] |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Brunetti F]] | ||
| + | [[Category: Ghiglione B]] | ||
| + | [[Category: Guardabassi L]] | ||
| + | [[Category: Gutkind G]] | ||
| + | [[Category: Klinke S]] | ||
| + | [[Category: Power P]] | ||
Current revision
Crystal structure of the class A carbapenemase CRH-1 in complex with avibactam at 1.4 Angstrom resolution
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