1iim

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[[Image:1iim.jpg|left|200px]]
 
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==thymidylyltransferase complexed with TTP==
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The line below this paragraph, containing "STRUCTURE_1iim", creates the "Structure Box" on the page.
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<StructureSection load='1iim' size='340' side='right'caption='[[1iim]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1iim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IIM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
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{{STRUCTURE_1iim| PDB=1iim | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iim OCA], [https://pdbe.org/1iim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iim RCSB], [https://www.ebi.ac.uk/pdbsum/1iim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iim ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RMLA_SALTY RMLA_SALTY] Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Is also able to convert non natural substrates such as a wide array of alpha-D-hexopyranosyl, deoxy-alpha-D-glucopyranosyl, aminodeoxy-alpha-D-hexopyranosyl and acetamidodeoxy-alpha-D-hexopyranosyl phosphates to their corresponding dTDP- and UDP-nucleotide sugars.<ref>PMID:8382158</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/1iim_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iim ConSurf].
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<div style="clear:both"></div>
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'''thymidylyltransferase complexed with TTP'''
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==See Also==
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*[[Glucose-1-phosphate thymidylyltransferase 3D structures|Glucose-1-phosphate thymidylyltransferase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Metabolite glycosylation is affected by three classes of enzymes: nucleotidylyltransferases, which activate sugars as nucleotide diphospho-derivatives, intermediate sugar-modifying enzymes and glycosyltransferases, which transfer the final derivatized activated sugars to aglycon substrates. One of the first crystal structures of an enzyme responsible for the first step in this cascade, alpha-D-glucopyranosyl phosphate thymidylyltransferase (Ep) from Salmonella, in complex with product (UDP-Glc) and substrate (dTTP) is reported at 2.0 A and 2.1 A resolution, respectively. These structures, in conjunction with the kinetic characterization of Ep, clarify the catalytic mechanism of this important enzyme class. Structure-based engineering of Ep produced modified enzymes capable of utilizing 'unnatural' sugar phosphates not accepted by wild type Ep. The demonstrated ability to alter nucleotidylyltransferase specificity by design is an integral component of in vitro glycosylation systems developed for the production of diverse glycorandomized libraries.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1IIM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IIM OCA].
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==Reference==
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Structure, mechanism and engineering of a nucleotidylyltransferase as a first step toward glycorandomization., Barton WA, Lesniak J, Biggins JB, Jeffrey PD, Jiang J, Rajashankar KR, Thorson JS, Nikolov DB, Nat Struct Biol. 2001 Jun;8(6):545-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11373625 11373625]
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[[Category: Glucose-1-phosphate thymidylyltransferase]]
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[[Category: Salmonella enterica]]
[[Category: Salmonella enterica]]
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[[Category: Single protein]]
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[[Category: Barton WA]]
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[[Category: Barton, W A.]]
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[[Category: Biggins JB]]
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[[Category: Biggins, J B.]]
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[[Category: Jeffrey PD]]
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[[Category: Jeffrey, P D.]]
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[[Category: Jiang J]]
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[[Category: Jiang, J.]]
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[[Category: Lesniak J]]
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[[Category: Lesniak, J.]]
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[[Category: Nikolov DB]]
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[[Category: Nikolov, D B.]]
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[[Category: Rajashankar KR]]
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[[Category: Rajashankar, K R.]]
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[[Category: Thorson JS]]
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[[Category: Thorson, J S.]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:02:43 2008''
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Current revision

thymidylyltransferase complexed with TTP

PDB ID 1iim

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