|
|
| (10 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1ik5.gif|left|200px]] | |
| | | | |
| - | <!--
| + | ==Crystal Structure of a 14mer RNA Containing Double UU Bulges in Two Crystal Forms: A Novel U*(AU) Intramolecular Base Triple== |
| - | The line below this paragraph, containing "STRUCTURE_1ik5", creates the "Structure Box" on the page.
| + | <StructureSection load='1ik5' size='340' side='right'caption='[[1ik5]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1ik5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IK5 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBR:5-BROMO-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>CBR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | {{STRUCTURE_1ik5| PDB=1ik5 | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ik5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ik5 OCA], [https://pdbe.org/1ik5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ik5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ik5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ik5 ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''Crystal Structure of a 14mer RNA Containing Double UU Bulges in Two Crystal Forms: A Novel U*(AU) Intramolecular Base Triple'''
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | ==Overview== | + | [[Category: Deng J]] |
| - | The RNA/DNA 14-mer, (gguauuucgguaCc)2 with consecutive uridine bulges (underlined) on each strand has been determined in two crystal forms, spermine bound (Sp-form) and spermine free (Sp-free). The former was solved by the MAD method with three-wavelength data collected at Brookhaven National Laboratory (BNL); the later isomorphous structure was solved by the molecular replacement method using data collected on our Raxis IIc imaging plate system. The two crystal forms belong to the space group C2 with one molecule of double-stranded 14 mer in the asymmetric unit. The Sp-form has cell constants, a = 60.06, b = 29.10, c = 52.57 A, beta = 120.79 degrees and was refined to 1.7 A resolution with a final Rwork/Rfree of 19.8%/22.7% using 8,549 independent reflections. The Sp-free structure has cell constants, a = 60.06, b = 29.58, c = 52.50 A, beta = 120.85 degrees and was refined to 1.8 A with a final Rwork/ Rfree of 20.8%/23.2% using 6,285 unique reflections. The two structures are identical, except that the Sp-form has a spermine bound in the major groove, parallel to the RNA helical axis. One of the uridine bulges forms a novel intramolecular U*(A x U) base triple. The helices are in the C3'-endo conformation (A-form), but the bulges adopt the C2'-endo sugar pucker. Furthermore, the bulges induce a kink (30 degrees) in the helix axis and a very large twist (55 degrees) between the base pairs flanking the bulges. The Sp-form has one Mg2+ ion whereas the Sp-free form has two Mg2+ ions.
| + | [[Category: Shi K]] |
| - | | + | [[Category: Sudarsanakumar C]] |
| - | ==About this Structure== | + | [[Category: Sundaralingam M]] |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IK5 OCA].
| + | [[Category: Xiong Y]] |
| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structures of two forms of a 14-mer RNA/DNA chimer duplex with double UU bulges: a novel intramolecular U*(A x U) base triple., Deng J, Xiong Y, Sudarsanakumar C, Shi K, Sundaralingam M, RNA. 2001 Oct;7(10):1425-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11680847 11680847]
| + | |
| - | [[Category: Deng, J.]]
| + | |
| - | [[Category: Shi, K.]] | + | |
| - | [[Category: Sudarsanakumar, C.]]
| + | |
| - | [[Category: Sundaralingam, M.]]
| + | |
| - | [[Category: Xiong, Y.]] | + | |
| - | [[Category: Base triple]] | + | |
| - | [[Category: Kink]] | + | |
| - | [[Category: Rna duplex]] | + | |
| - | [[Category: Uu-bulge]] | + | |
| - | [[Category: X-ray structure]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:05:23 2008''
| + | |
