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4eyy
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4eyy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_str._Corby Legionella pneumophila str. Corby] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EYY FirstGlance]. <br> | <table><tr><td colspan='2'>[[4eyy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_str._Corby Legionella pneumophila str. Corby] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EYY FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eyy OCA], [https://pdbe.org/4eyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eyy RCSB], [https://www.ebi.ac.uk/pdbsum/4eyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eyy ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eyy OCA], [https://pdbe.org/4eyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eyy RCSB], [https://www.ebi.ac.uk/pdbsum/4eyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eyy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/Q5ZYC9_LEGPH Q5ZYC9_LEGPH] | [https://www.uniprot.org/uniprot/Q5ZYC9_LEGPH Q5ZYC9_LEGPH] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | A Type 4b secretion system (T4bSS) is required for Legionella growth in alveolar macrophages. IcmQ associates with IcmR, binds to membranes, and has a critical role in the T4bSS. We have now solved a crystal structure of IcmR-IcmQ to further our understanding of this complex. This structure revealed an amphipathic four-helix bundle, formed by IcmR and the N-terminal domain of IcmQ, which is linked to a novel C-terminal domain of IcmQ (Qc) by a linker helix. The Qc domain has structural homology with ADP ribosyltransferase domains in certain bacterial toxins and binds NAD(+) with a dissociation constant in the physiological range. Structural homology and molecular dynamics were used to identify an extended NAD(+) binding site on Qc, and the resulting model was tested by mutagenesis and binding assays. Based on the data, we suggest that IcmR-IcmQ binds to membranes, where it may interact with, or perhaps modify, a protein in the T4bSS when NAD(+) is bound. | ||
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| - | IcmQ in the Type 4b secretion system contains an NAD+ binding domain.,Farelli JD, Gumbart JC, Akey IV, Hempstead A, Amyot W, Head JF, McKnight CJ, Isberg RR, Akey CW Structure. 2013 Aug 6;21(8):1361-73. doi: 10.1016/j.str.2013.05.017. Epub 2013, Jul 11. PMID:23850453<ref>PMID:23850453</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4eyy" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal Structure of the IcmR-IcmQ complex from Legionella pneumophila
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