4f1n

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1n OCA], [https://pdbe.org/4f1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f1n RCSB], [https://www.ebi.ac.uk/pdbsum/4f1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1n ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The RNA-induced silencing complex, comprising Argonaute and guide RNA, mediates RNA interference. Here we report the 3.2 A crystal structure of Kluyveromyces polysporus Argonaute (KpAGO) fortuitously complexed with guide RNA originating from small-RNA duplexes autonomously loaded by recombinant KpAGO. Despite their diverse sequences, guide-RNA nucleotides 1-8 are positioned similarly, with sequence-independent contacts to bases, phosphates and 2'-hydroxyl groups pre-organizing the backbone of nucleotides 2-8 in a near-A-form conformation. Compared with prokaryotic Argonautes, KpAGO has numerous surface-exposed insertion segments, with a cluster of conserved insertions repositioning the N domain to enable full propagation of guide-target pairing. Compared with Argonautes in inactive conformations, KpAGO has a hydrogen-bond network that stabilizes an expanded and repositioned loop, which inserts an invariant glutamate into the catalytic pocket. Mutation analyses and analogies to ribonuclease H indicate that insertion of this glutamate finger completes a universally conserved catalytic tetrad, thereby activating Argonaute for RNA cleavage.

Structure of yeast Argonaute with guide RNA.,Nakanishi K, Weinberg DE, Bartel DP, Patel DJ Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211. PMID:22722195<ref>PMID:22722195</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

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