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From Proteopedia
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== Uracil-DNA Glycosylase == | == Uracil-DNA Glycosylase == | ||
The structure of Glycosylase has a couple of different forms in terms of its general structure there is Adenine and Uracil Glycosylase. DNA Uracil-Glycosylase specifically looks for any Uracil in the double-stranded DNA. It looks for Uracil in dsDNA because uracil is only found in RNA. So if a Uracil is found in dsDNA then that means one of the strands has been damaged and needs repair. The dsDNA in the 3D model contains a U G base pair mismatch. When Uracil-DNA Glycosylase finds the <scene name='92/927197/Uracil/4'>Uracil</scene> site it binds to it. Then a nucleotide-flipping mechanism flips the site of repair out of the double helix. The<scene name='92/927197/Active_site/7'>Active Site</scene> of Uracil Glycosylase; D145, Y147, F158, N204, H268, L272 is what binds to the double-stranded DNA with the damaged lesion. This is what allows the <scene name='92/927197/Uracil_glycolysis_interaction/5'>Uracil_Glycosylase interaction</scene> and flipping of the damaged site out of the double helix. ASN 204 and HIS 268 are responsible for catalyzing the cleavage of the glycosidic bond. TYR 147, PHE 158, and ASN 204 all aid in Uracil excision and replacement with Thymine. When flipped the damaged bases out of the helix <scene name='92/927197/Arg_side_chain/1'>ARG 272 side chain</scene> takes its place in the minor groove since AP sites can be mutagenic<ref>PMID:8900285</ref>. The Uracil is then replaced with a Thymine. This is because Uracil and Thymine have identical base pairing properties. Thymine happens to have greater resistance to photochemical mutations which is why we see it in dsDNA and not Uracil. | The structure of Glycosylase has a couple of different forms in terms of its general structure there is Adenine and Uracil Glycosylase. DNA Uracil-Glycosylase specifically looks for any Uracil in the double-stranded DNA. It looks for Uracil in dsDNA because uracil is only found in RNA. So if a Uracil is found in dsDNA then that means one of the strands has been damaged and needs repair. The dsDNA in the 3D model contains a U G base pair mismatch. When Uracil-DNA Glycosylase finds the <scene name='92/927197/Uracil/4'>Uracil</scene> site it binds to it. Then a nucleotide-flipping mechanism flips the site of repair out of the double helix. The<scene name='92/927197/Active_site/7'>Active Site</scene> of Uracil Glycosylase; D145, Y147, F158, N204, H268, L272 is what binds to the double-stranded DNA with the damaged lesion. This is what allows the <scene name='92/927197/Uracil_glycolysis_interaction/5'>Uracil_Glycosylase interaction</scene> and flipping of the damaged site out of the double helix. ASN 204 and HIS 268 are responsible for catalyzing the cleavage of the glycosidic bond. TYR 147, PHE 158, and ASN 204 all aid in Uracil excision and replacement with Thymine. When flipped the damaged bases out of the helix <scene name='92/927197/Arg_side_chain/1'>ARG 272 side chain</scene> takes its place in the minor groove since AP sites can be mutagenic<ref>PMID:8900285</ref>. The Uracil is then replaced with a Thymine. This is because Uracil and Thymine have identical base pairing properties. Thymine happens to have greater resistance to photochemical mutations which is why we see it in dsDNA and not Uracil. | ||
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== References == | == References == | ||
<references/> | <references/> | ||
Current revision
DNA Repair Mechanism; URACIL-DNA GLYCOSYLASE
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