8b5e
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Exploring the ligand binding and conformational dynamics of receptor domain 1 of the ABC transporter GlnPQ== | |
| + | <StructureSection load='8b5e' size='340' side='right'caption='[[8b5e]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8b5e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B5E FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b5e OCA], [https://pdbe.org/8b5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b5e RCSB], [https://www.ebi.ac.uk/pdbsum/8b5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b5e ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A2X0R690_9LACT A0A2X0R690_9LACT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The adenosine triphosphate (ATP)-binding cassette (ABC) importer GlnPQ from Lactococcus lactis has two sequential covalently linked substrate-binding domains (SBDs), which capture the substrates and deliver them to the translocon. The two SBDs differ in their ligand specificities, binding affinities and the distance to the transmembrane domain; interestingly, both SBDs can bind their ligands simultaneously without affecting each other. In this work, we studied the binding of ligands to both SBDs using X-ray crystallography and molecular dynamics simulations. We report three high-resolution structures of SBD1, namely, the wild-type SBD1 with bound asparagine or arginine, and E184D SBD1 with glutamine bound. Molecular dynamics (MD) simulations provide a detailed insight into the dynamics associated with open-closed transitions of the SBDs. | ||
| - | + | Exploring the Ligand Binding and Conformational Dynamics of the Substrate-Binding Domain 1 of the ABC Transporter GlnPQ.,Nemchinova M, Schuurman-Wolters GK, Whittaker JJ, Arkhipova V, Marrink SJ, Poolman B, Guskov A J Phys Chem B. 2024 Aug 15;128(32):7822-7832. doi: 10.1021/acs.jpcb.4c02662. Epub , 2024 Aug 1. PMID:39090964<ref>PMID:39090964</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Guskov | + | <div class="pdbe-citations 8b5e" style="background-color:#fffaf0;"></div> |
| - | [[Category: Whittaker | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Lactococcus lactis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Guskov A]] | ||
| + | [[Category: Whittaker J]] | ||
Current revision
Exploring the ligand binding and conformational dynamics of receptor domain 1 of the ABC transporter GlnPQ
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