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8b9d
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8b9d is ON HOLD Authors: Jones, M.L., Yeeles, J.T.P. Description: Human replisome bound by Pol Alpha Category: Unreleased Structures [[Category...) |
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| - | '''Unreleased structure''' | ||
| - | The entry | + | ==Human replisome bound by Pol Alpha== |
| + | <StructureSection load='8b9d' size='340' side='right'caption='[[8b9d]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8b9d]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B9D FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b9d OCA], [https://pdbe.org/8b9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b9d RCSB], [https://www.ebi.ac.uk/pdbsum/8b9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b9d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MCM2_HUMAN MCM2_HUMAN] Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.<ref>PMID:8175912</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | During eukaryotic DNA replication, Pol alpha-primase generates primers at replication origins to start leading-strand synthesis and every few hundred nucleotides during discontinuous lagging-strand replication. How Pol alpha-primase is targeted to replication forks to prime DNA synthesis is not fully understood. Here, by determining cryoelectron microscopy (cryo-EM) structures of budding yeast and human replisomes containing Pol alpha-primase, we reveal a conserved mechanism for the coordination of priming by the replisome. Pol alpha-primase binds directly to the leading edge of the CMG (CDC45-MCM-GINS) replicative helicase via a complex interaction network. The non-catalytic PRIM2/Pri2 subunit forms two interfaces with CMG that are critical for in vitro DNA replication and yeast cell growth. These interactions position the primase catalytic subunit PRIM1/Pri1 directly above the exit channel for lagging-strand template single-stranded DNA (ssDNA), revealing why priming occurs efficiently only on the lagging-strand template and elucidating a mechanism for Pol alpha-primase to overcome competition from RPA to initiate primer synthesis. | ||
| - | + | How Pol alpha-primase is targeted to replisomes to prime eukaryotic DNA replication.,Jones ML, Aria V, Baris Y, Yeeles JTP Mol Cell. 2023 Jul 27:S1097-2765(23)00511-7. doi: 10.1016/j.molcel.2023.06.035. PMID:37506699<ref>PMID:37506699</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Jones | + | <div class="pdbe-citations 8b9d" style="background-color:#fffaf0;"></div> |
| - | [[Category: Yeeles | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Jones ML]] | ||
| + | [[Category: Yeeles JTP]] | ||
Current revision
Human replisome bound by Pol Alpha
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