8h40

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'''Unreleased structure'''
 
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The entry 8h40 is ON HOLD
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==Cryo-EM structure of the transcription activation complex NtcA-TAC==
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<StructureSection load='8h40' size='340' side='right'caption='[[8h40]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8h40]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena Anabaena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H40 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h40 OCA], [https://pdbe.org/8h40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h40 RCSB], [https://www.ebi.ac.uk/pdbsum/8h40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h40 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOB_NOSS1 RPOB_NOSS1] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01321]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transcription factors respond to multilevel stimuli and co-occupy promoter regions of target genes to activate RNA polymerase (RNAP) in a cooperative manner. To decipher the molecular mechanism, here we report two cryo-electron microscopy structures of Anabaena transcription activation complexes (TACs): NtcA-TAC composed of RNAP holoenzyme, promoter and a global activator NtcA, and NtcA-NtcB-TAC comprising an extra context-specific regulator, NtcB. Structural analysis showed that NtcA binding makes the promoter DNA bend by approximately 50 degrees , which facilitates RNAP to contact NtcB at the distal upstream NtcB box. The sequential binding of NtcA and NtcB induces looping back of promoter DNA towards RNAP, enabling the assembly of a fully activated TAC bound with two activators. Together with biochemical assays, we propose a 'DNA looping' mechanism of cooperative transcription activation in bacteria.
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Authors: Han, S.J., Jiang, Y.L., You, L.L., Shen, L.Q., Yang, F., Kong, W.W., Chen, Z.P., Zhang, Y., Zhou, C.Z.
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DNA looping mediates cooperative transcription activation.,Han SJ, Jiang YL, You LL, Shen LQ, Wu X, Yang F, Cui N, Kong WW, Sun H, Zhou K, Meng HC, Chen ZP, Chen Y, Zhang Y, Zhou CZ Nat Struct Mol Biol. 2024 Feb;31(2):293-299. doi: 10.1038/s41594-023-01149-7. , Epub 2024 Jan 4. PMID:38177666<ref>PMID:38177666</ref>
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Description: Cryo-EM structure of the transcription activation complex NtcA-TAC
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Chen, Z.P]]
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<div class="pdbe-citations 8h40" style="background-color:#fffaf0;"></div>
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[[Category: Han, S.J]]
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[[Category: Zhang, Y]]
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==See Also==
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[[Category: Jiang, Y.L]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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[[Category: You, L.L]]
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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[[Category: Yang, F]]
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== References ==
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[[Category: Zhou, C.Z]]
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<references/>
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[[Category: Kong, W.W]]
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__TOC__
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[[Category: Shen, L.Q]]
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</StructureSection>
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[[Category: Anabaena]]
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[[Category: Large Structures]]
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[[Category: Chen ZP]]
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[[Category: Han SJ]]
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[[Category: Jiang YL]]
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[[Category: Kong WW]]
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[[Category: Shen LQ]]
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[[Category: Wu XX]]
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[[Category: Yang F]]
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[[Category: You LL]]
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[[Category: Zhang Y]]
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[[Category: Zhou CZ]]

Current revision

Cryo-EM structure of the transcription activation complex NtcA-TAC

PDB ID 8h40

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