8h5o

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'''Unreleased structure'''
 
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The entry 8h5o is ON HOLD until Paper Publication
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==Crystal structure of PETase S121E/P181V/D186H/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis==
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<StructureSection load='8h5o' size='340' side='right'caption='[[8h5o]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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Authors: Lee, S.H., Seo, H., Kim, K.-J.
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8h5o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ideonella_sakaiensis Ideonella sakaiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H5O FirstGlance]. <br>
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Description: Crystal structure of PETase S121E/P181V/D186H/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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[[Category: Unreleased Structures]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h5o OCA], [https://pdbe.org/8h5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h5o RCSB], [https://www.ebi.ac.uk/pdbsum/8h5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h5o ProSAT]</span></td></tr>
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[[Category: Kim, K.-J]]
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</table>
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[[Category: Seo, H]]
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== Function ==
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[[Category: Lee, S.H]]
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[https://www.uniprot.org/uniprot/PETH_PISS1 PETH_PISS1] Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Likely acts synergistically with MHETase to depolymerize PET (PubMed:26965627). Catalyzes the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product (PubMed:26965627, PubMed:29235460, PubMed:29374183, PubMed:29603535, PubMed:29666242, PubMed:32269349). Also depolymerizes another semiaromatic polyester, poly(ethylene-2,5-furandicarboxylate) (PEF), which is an emerging, bioderived PET replacement with improved gas barrier properties (PubMed:29666242). In contrast, PETase does not degrade aliphatic polyesters such as polylactic acid (PLA) and polybutylene succinate (PBS) (PubMed:29666242). Is also able to hydrolyze bis(hydroxyethyl) terephthalate (BHET) to yield MHET with no further decomposition, but terephthalate (TPA) can also be observed (PubMed:26965627, PubMed:29374183, PubMed:29603535). Shows esterase activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) in vitro (PubMed:26965627, PubMed:30502092).<ref>PMID:26965627</ref> <ref>PMID:29235460</ref> <ref>PMID:29374183</ref> <ref>PMID:29603535</ref> <ref>PMID:29666242</ref> <ref>PMID:30502092</ref> <ref>PMID:32269349</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Ideonella sakaiensis]]
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[[Category: Large Structures]]
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[[Category: Kim K-J]]
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[[Category: Lee SH]]
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[[Category: Seo H]]

Current revision

Crystal structure of PETase S121E/P181V/D186H/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis

PDB ID 8h5o

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