4gkl
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4gkl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_neapolitana Thermotoga neapolitana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GKL FirstGlance]. <br> | <table><tr><td colspan='2'>[[4gkl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_neapolitana Thermotoga neapolitana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GKL FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gkl OCA], [https://pdbe.org/4gkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gkl RCSB], [https://www.ebi.ac.uk/pdbsum/4gkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gkl ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gkl OCA], [https://pdbe.org/4gkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gkl RCSB], [https://www.ebi.ac.uk/pdbsum/4gkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gkl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/B5ARZ9_THENE B5ARZ9_THENE] | [https://www.uniprot.org/uniprot/B5ARZ9_THENE B5ARZ9_THENE] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | An intracellular alpha-amylase, AmyB, has been cloned from the hyperthermophilic bacterium Thermotoga neapolitana. AmyB belongs to glycoside hydrolase family 13 and liberates maltose from diverse substrates, including starch, amylose, amylopectin and glycogen. The final product of AmyB is similar to that of typical maltogenic amylases, but AmyB cleaves maltose units from the nonreducing end, which is a unique property of this alpha-amylase. In this study, the crystal structure of AmyB from T. neapolitana has been determined at 2.4 A resolution, revealing that the monomeric AmyB comprises domains A, B and C like other alpha-amylases, but with structural variations. In the structure, a wider active site and a putative extra sugar-binding site at the top of the active site were found. Subsequent biochemical results suggest that the extra sugar-binding site is suitable for recognizing the nonreducing end of the substrates, explaining the unique activity of this enzyme. These findings provide a structural basis for the ability of an alpha-amylase that has the common alpha-amylase structure to show a diverse substrate specificity. | ||
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| - | Structure of a novel alpha-amylase AmyB from Thermotoga neapolitana that produces maltose from the nonreducing end of polysaccharides.,Jun SY, Kim JS, Choi KH, Cha J, Ha NC Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):442-50. doi:, 10.1107/S0907444912049219. Epub 2013 Feb 16. PMID:23519419<ref>PMID:23519419</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4gkl" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Amylase 3D structures|Amylase 3D structures]] | *[[Amylase 3D structures|Amylase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of a noncanonic maltogenic alpha-amylase AmyB from Thermotoga neapolitana
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