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8bgw
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8bgw is ON HOLD Authors: Flynn, A., Antonyuk, S.V., Eady, R.R., Muench, S.P., Hasnain, S.S. Description: CryoEM structure of quinol-dependent Nitri...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of quinol-dependent Nitric Oxide Reductase (qNOR) from Alcaligenes xylosoxidans at 2.2 A resolution== | |
| + | <StructureSection load='8bgw' size='340' side='right'caption='[[8bgw]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8bgw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BGW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=10M:DECYL+4-O-ALPHA-D-GLUCOPYRANOSYL-1-THIO-BETA-D-GLUCOPYRANOSIDE'>10M</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=LOP:(1R)-2-{[(R)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(DODECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>LOP</scene>, <scene name='pdbligand=UQ1:UBIQUINONE-1'>UQ1</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bgw OCA], [https://pdbe.org/8bgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bgw RCSB], [https://www.ebi.ac.uk/pdbsum/8bgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bgw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0D6H8R3_ALCXX A0A0D6H8R3_ALCXX] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Quinol-dependent nitric oxide reductases (qNORs) are considered members of the respiratory heme-copper oxidase superfamily, are unique to bacteria, and are commonly found in pathogenic bacteria where they play a role in combating the host immune response. qNORs are also essential enzymes in the denitrification pathway, catalysing the reduction of nitric oxide to nitrous oxide. Here, we determine a 2.2 A cryoEM structure of qNOR from Alcaligenes xylosoxidans, an opportunistic pathogen and a denitrifying bacterium of importance in the nitrogen cycle. This high-resolution structure provides insight into electron, substrate, and proton pathways, and provides evidence that the quinol binding site not only contains the conserved His and Asp residues but also possesses a critical Arg (Arg720) observed in cytochrome bo(3), a respiratory quinol oxidase. | ||
| - | + | A 2.2 A cryoEM structure of a quinol-dependent NO Reductase shows close similarity to respiratory oxidases.,Flynn AJ, Antonyuk SV, Eady RR, Muench SP, Hasnain SS Nat Commun. 2023 Jun 9;14(1):3416. doi: 10.1038/s41467-023-39140-x. PMID:37296134<ref>PMID:37296134</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8bgw" style="background-color:#fffaf0;"></div> |
| - | [[Category: Antonyuk | + | == References == |
| - | [[Category: Eady | + | <references/> |
| - | [[Category: Flynn | + | __TOC__ |
| - | [[Category: Hasnain | + | </StructureSection> |
| + | [[Category: Achromobacter xylosoxidans]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Antonyuk SV]] | ||
| + | [[Category: Eady RR]] | ||
| + | [[Category: Flynn A]] | ||
| + | [[Category: Hasnain SS]] | ||
| + | [[Category: Muench SP]] | ||
Current revision
CryoEM structure of quinol-dependent Nitric Oxide Reductase (qNOR) from Alcaligenes xylosoxidans at 2.2 A resolution
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