1j25

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[[Image:1j25.jpg|left|200px]]
 
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==Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain, Mn cocrystal==
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The line below this paragraph, containing "STRUCTURE_1j25", creates the "Structure Box" on the page.
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<StructureSection load='1j25' size='340' side='right'caption='[[1j25]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1j25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J25 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_1j25| PDB=1j25 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j25 OCA], [https://pdbe.org/1j25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j25 RCSB], [https://www.ebi.ac.uk/pdbsum/1j25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j25 ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain, Mn cocrystal'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q8TZH8_PYRFU Q8TZH8_PYRFU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. Here, we report the domain organization of an archaeal homolog (Hef) of this family and the X-ray crystal structure of the middle domain, with the nuclease activity. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases, including the correspondence of the GDX(n)ERKX(3)D signature motif in Hef to the PDX(n)(E/D)XK motif in restriction enzymes. This structural study also suggests that the XPF/Rad1/Mus81/ERCC1 proteins form a dimer through each interface of the nuclease domain and the helix-hairpin-helix domain. Simultaneous disruptions of both interfaces result in their dissociation into separate monomers, with strikingly reduced endonuclease activities.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j2/1j25_consurf.spt"</scriptWhenChecked>
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1J25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J25 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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X-ray and biochemical anatomy of an archaeal XPF/Rad1/Mus81 family nuclease: similarity between its endonuclease domain and restriction enzymes., Nishino T, Komori K, Ishino Y, Morikawa K, Structure. 2003 Apr;11(4):445-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12679022 12679022]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j25 ConSurf].
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[[Category: Pyrococcus furiosus dsm 3638]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Ishino, Y.]]
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</StructureSection>
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[[Category: Komori, K.]]
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[[Category: Large Structures]]
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[[Category: Morikawa, K.]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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[[Category: Nishino, T.]]
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[[Category: Ishino Y]]
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[[Category: Structure-specific endonuclease]]
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[[Category: Komori K]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:42:54 2008''
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[[Category: Morikawa K]]
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[[Category: Nishino T]]

Current revision

Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain, Mn cocrystal

PDB ID 1j25

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