8f27
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Mirror-image DNA containing 2'-OMe-L-uridine residue== | |
| + | <StructureSection load='8f27' size='340' side='right'caption='[[8f27]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8f27]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8F27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8F27 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0DA:2-DEOXY-L-RIBO-FURANOSYL+ADENOSINE-5-MONOPHOSPHATE'>0DA</scene>, <scene name='pdbligand=0DC:2-DEOXY-L-RIBO-FURANOSYL+CYTOSINE-5-MONOPHOSPHATE'>0DC</scene>, <scene name='pdbligand=0DG:2-DEOXY-L-RIBO-FURANOSYL+GUANINE-5-MONOPHOSPHATE'>0DG</scene>, <scene name='pdbligand=0DT:2-DEOXY-L-RIBO-FURANOSYL+THYMIDINE-5-MONOPHOSPHATE'>0DT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XEC:1-(2-O-methyl-5-O-phosphono-beta-L-ribofuranosyl)pyrimidine-2,4(1H,3H)-dione'>XEC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8f27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8f27 OCA], [https://pdbe.org/8f27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8f27 RCSB], [https://www.ebi.ac.uk/pdbsum/8f27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8f27 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | To further expand the functionality and enhance the stability of mirror-image nucleic acid as advanced agent for basic research and therapeutic design, we have synthesized the 2'-deoxy2'-methoxy-L-uridine phosphoramidites and incorporate it into L-DNA and L-RNA via solid-phase synthesis quantitatively. We found that the thermostability of L-nucleic acids is dramatically improved after introducing the modifications. Moreover, we successfully crystallized both L-DNA and L-RNA duplexes containing the 2'-OMe modifications and sharing the identical sequence. The crystal structure determination and analysis reveal the overall structures of the mirrorimage nucleic acids, and for the first time interpretate the structural deviation caused by 2'-OMe and 2'-OH groups in the oligonucleotides which are highly similar. This novel chemical modification of nucleic acid has the potential to be used to design nucleic acid-based therapeutic and material in the future. | ||
| - | + | Derivatization of Mirror-image L-Nucleic Acids with 2'-OMe Modification for Thermal and Structural Stabilization.,Dantsu Y, Zhang W Chembiochem. 2023 Mar 9:e202200764. doi: 10.1002/cbic.202200764. PMID:36892526<ref>PMID:36892526</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Dantsu | + | <div class="pdbe-citations 8f27" style="background-color:#fffaf0;"></div> |
| - | [[Category: Zhang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Dantsu Y]] | ||
| + | [[Category: Zhang W]] | ||
Current revision
Mirror-image DNA containing 2'-OMe-L-uridine residue
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