7sr3

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7sr3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Human_papillomavirus_type_16 Human papillomavirus type 16], [https://en.wikipedia.org/wiki/Lama_glama Lama glama] and [https://en.wikipedia.org/wiki/Pongo_pygmaeus Pongo pygmaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SR3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[7sr3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Human_papillomavirus_type_16 Human papillomavirus type 16], [https://en.wikipedia.org/wiki/Lama_glama Lama glama] and [https://en.wikipedia.org/wiki/Pongo_pygmaeus Pongo pygmaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SR3 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sr3 OCA], [https://pdbe.org/7sr3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sr3 RCSB], [https://www.ebi.ac.uk/pdbsum/7sr3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sr3 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sr3 OCA], [https://pdbe.org/7sr3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sr3 RCSB], [https://www.ebi.ac.uk/pdbsum/7sr3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sr3 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/VE7_HPV16 VE7_HPV16] Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).[HAMAP-Rule:MF_04004]<ref>PMID:11080488</ref> <ref>PMID:1316611</ref> <ref>PMID:2836062</ref> [https://www.uniprot.org/uniprot/A0A678ZGP6_HUMAN A0A678ZGP6_HUMAN] [https://www.uniprot.org/uniprot/B2MG_PONPY B2MG_PONPY] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system (By similarity).
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== Publication Abstract from PubMed ==
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MHC class I "single-chain trimer" molecules, coupling MHC heavy chain, beta(2)-microglobulin, and a specific peptide into a single polypeptide chain, are widely used in research. To more fully understand caveats associated with this design that may affect its use for basic and translational studies, we evaluated a set of engineered single-chain trimers with combinations of stabilizing mutations across eight different classical and non-classical human class I alleles with 44 different peptides, including a novel human/murine chimeric design. While, overall, single-chain trimers accurately recapitulate native molecules, care was needed in selecting designs for studying peptides longer or shorter than 9-mers, as single-chain trimer design could affect peptide conformation. In the process, we observed that predictions of peptide binding were often discordant with experiment and that yields and stabilities varied widely with construct design. We also developed novel reagents to improve the crystallizability of these proteins and confirmed novel modes of peptide presentation.
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Effects of HLA single chain trimer design on peptide presentation and stability.,Finton KAK, Rupert PB, Friend DJ, Dinca A, Lovelace ES, Buerger M, Rusnac DV, Foote-McNabb U, Chour W, Heath JR, Campbell JS, Pierce RH, Strong RK Front Immunol. 2023 May 3;14:1170462. doi: 10.3389/fimmu.2023.1170462. , eCollection 2023. PMID:37207206<ref>PMID:37207206</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7sr3" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[MHC 3D structures|MHC 3D structures]]
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*[[MHC I 3D structures|MHC I 3D structures]]
== References ==
== References ==
<references/>
<references/>

Current revision

Single chain trimer HLA-A*02:01 (H98L, Y108C) with HPV.16 E7 peptide YMLDLQPETTDL

PDB ID 7sr3

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