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4j7y

From Proteopedia

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Current revision

Human LTC4 synthase in complex with product analogs - implications for enzyme catalysis

Structural highlights

4j7y is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.901Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

LTC4S_HUMAN Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency) [MIM:246530. LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.

Function

LTC4S_HUMAN Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.

Publication Abstract from PubMed

Leukotriene (LT) C4 synthase (LTC4S) catalyzes the conjugation of the fatty acid LTA4 with the tripeptide GSH to produce LTC4, the parent compound of the cysteinyl-leukotrienes, important mediators of asthma. Here we mutated Trp116 in human LTC4S, a residue proposed to play a key role in substrate binding, into an Ala or Phe. Biochemical and structural characterization of these mutants along with crystal structures of the wild type and mutated enzymes in complex with three product analogs, viz. S-hexyl-, 4- phenyl-butyl-, and 2-hydroxy-4-phenyl-butyl-glutathione, provide new insights to binding of substrates and product, identifies a new conformation of the GSH moiety at the active site, and suggests a route for product release, aided by Trp116.

Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism.,Niegowski D, Kleinschmidt T, Olsson U, Ahmad S, Rinaldo-Matthis A, Haeggstrom JZ J Biol Chem. 2013 Dec 23. PMID:24366866^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Niegowski D, Kleinschmidt T, Olsson U, Ahmad S, Rinaldo-Matthis A, Haeggstrom JZ. Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism. J Biol Chem. 2013 Dec 23. PMID:24366866 doi:http://dx.doi.org/10.1074/jbc.M113.534628

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4j7y"

Categories: Homo sapiens | Large Structures | Haeggstrom JZ | Niegowski D | Rinaldo-Matthis A

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4j7y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J7Y FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1JP:D-GAMMA-GLUTAMYL-(Z)-N-(CARBOXYMETHYLIDENE)-S-[(2R)-2-HYDROXY-4-PHENYLBUTYL]-L-CYSTEINAMIDE'>1JP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.901&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1JP:D-GAMMA-GLUTAMYL-(Z)-N-(CARBOXYMETHYLIDENE)-S-[(2R)-2-HYDROXY-4-PHENYLBUTYL]-L-CYSTEINAMIDE'>1JP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7y OCA], [https://pdbe.org/4j7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j7y RCSB], [https://www.ebi.ac.uk/pdbsum/4j7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7y ProSAT]</span></td></tr>
 </table>

4j7y

From Proteopedia

Current revision

Human LTC4 synthase in complex with product analogs - implications for enzyme catalysis

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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