1hlv

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(New page: 200px<br /> <applet load="1hlv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hlv, resolution 2.5&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1hlv.gif|left|200px]]<br />
 
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<applet load="1hlv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hlv, resolution 2.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CENP-B(1-129) COMPLEXED WITH THE CENP-B BOX DNA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CENP-B(1-129) COMPLEXED WITH THE CENP-B BOX DNA==
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The human centromere protein B (CENP-B), one of the centromere components, specifically binds a 17 bp sequence (the CENP-B box), which appears in, every other alpha-satellite repeat. In the present study, the crystal, structure of the complex of the DNA-binding region (129 residues) of, CENP-B and the CENP-B box DNA has been determined at 2.5 A resolution. The, DNA-binding region forms two helix-turn-helix domains, which are bound to, adjacent major grooves of the DNA. The DNA is kinked at the two, recognition helix contact sites, and the DNA region between the kinks is, straight. Among the major groove protein-bound DNAs, this, 'kink-straight-kink' bend contrasts with ordinary 'round bends' (gradual, bending between two protein contact sites). The larger kink (43 degrees ), is induced by a novel mechanism, 'phosphate bridging by an arginine-rich, helix': the recognition helix with an arginine cluster is inserted, perpendicularly into the major groove and bridges the groove through, direct interactions with the phosphate groups. The overall bending angle, is 59 degrees, which may be important for the centromere-specific, chromatin structure.
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<StructureSection load='1hlv' size='340' side='right'caption='[[1hlv]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hlv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HLV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hlv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hlv OCA], [https://pdbe.org/1hlv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hlv RCSB], [https://www.ebi.ac.uk/pdbsum/1hlv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hlv ProSAT], [https://www.topsan.org/Proteins/RSGI/1hlv TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CENPB_HUMAN CENPB_HUMAN] Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/1hlv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hlv ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1HLV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HLV OCA].
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*[[Centromere protein 3D structure|Centromere protein 3D structure]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA., Tanaka Y, Nureki O, Kurumizaka H, Fukai S, Kawaguchi S, Ikuta M, Iwahara J, Okazaki T, Yokoyama S, EMBO J. 2001 Dec 3;20(23):6612-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11726497 11726497]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fukai, S.]]
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[[Category: Fukai S]]
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[[Category: Ikuta, M.]]
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[[Category: Ikuta M]]
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[[Category: Iwahara, J.]]
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[[Category: Iwahara J]]
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[[Category: Kawaguchi, S.]]
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[[Category: Kawaguchi S]]
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[[Category: Kurumizaka, H.]]
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[[Category: Kurumizaka H]]
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[[Category: Nureki, O.]]
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[[Category: Nureki O]]
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[[Category: Okazaki, T.]]
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[[Category: Okazaki T]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Tanaka Y]]
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[[Category: Tanaka, Y.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: helix-turn-helix]]
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[[Category: protein-dna complex]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:20:03 2007''
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Current revision

CRYSTAL STRUCTURE OF CENP-B(1-129) COMPLEXED WITH THE CENP-B BOX DNA

PDB ID 1hlv

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