1hph

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1hph" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hph" /> '''STRUCTURE OF HUMAN PARATHYROID HORMONE 1-37...)
Current revision (11:34, 27 March 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1hph.gif|left|200px]]<br />
 
-
<applet load="1hph" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1hph" />
 
-
'''STRUCTURE OF HUMAN PARATHYROID HORMONE 1-37 IN SOLUTION'''<br />
 
-
==Overview==
+
==STRUCTURE OF HUMAN PARATHYROID HORMONE 1-37 IN SOLUTION==
-
Human parathyroid hormone (hPTH), amino acids Ser1 to Leu37, is, biologically active with respect to both receptor binding and activation, of adenylate cyclase to influence the serum calcium concentration. It, induces DNA synthesis via an unknown signal pathway. We investigated the, structure of hPTH(1-37) in H2O/buffer solution under near physiological, conditions, that is pH 6.0 and 270 mM salt, by circular dichroism, ultracentrifugation, nuclear magnetic resonance spectroscopy, and, molecular dynamics calculations. Complete sequence specific assignments of, all 1H resonances were performed by using 1H two-dimensional NMR, measurements (double quantum-filtered correlated spectroscopy, nuclear, Overhauser effect spectroscopy (NOESY), and total correlation spectroscopy, with suppression of NOESY-type cross-peaks spectra). hPTH(1-37) obtained, helical structure and showed hydrophobic interactions defining a tertiary, structure. The NH2-terminal four amino acids of hPTH(1-37) did not show a, stable conformation. Evidence for an alpha-helical region between Ile5 and, Asn10 was found. This region was followed by a flexible link (Gly12, Lys13) and a well defined turn region, His14 to Ser17. The latter was, stabilized by hydrophobic interactions between Trp23 and Leu15. Ser17, through at least Leu28 formed an alpha-helix. Arg20 and Lys27 were, involved in the core built by His14 to Ser17. Unrestrained molecular, dynamics simulations indicated that the structure was stable on the 200 ps, time scale.
+
<StructureSection load='1hph' size='340' side='right'caption='[[1hph]]' scene=''>
-
 
+
== Structural highlights ==
-
==Disease==
+
<table><tr><td colspan='2'>[[1hph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HPH FirstGlance]. <br>
-
Known diseases associated with this structure: Hypoparathyroidism, autosomal dominant OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=168450 168450]], Hypoparathyroidism, autosomal recessive OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=168450 168450]]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hph OCA], [https://pdbe.org/1hph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hph RCSB], [https://www.ebi.ac.uk/pdbsum/1hph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hph ProSAT]</span></td></tr>
-
==About this Structure==
+
</table>
-
1HPH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HPH OCA].
+
== Disease ==
-
 
+
[https://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN] Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:[https://omim.org/entry/146200 146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.<ref>PMID:2212001</ref> <ref>PMID:10523031</ref> <ref>PMID:18056632</ref>
-
==Reference==
+
== Function ==
-
Structure of human parathyroid hormone 1-37 in solution., Marx UC, Austermann S, Bayer P, Adermann K, Ejchart A, Sticht H, Walter S, Schmid FX, Jaenicke R, Forssmann WG, et al., J Biol Chem. 1995 Jun 23;270(25):15194-202. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7797503 7797503]
+
[https://www.uniprot.org/uniprot/PTHY_HUMAN PTHY_HUMAN] PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.<ref>PMID:21076856</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/1hph_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hph ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Marx, U.C.]]
+
[[Category: Marx UC]]
-
[[Category: Roesch, P.]]
+
[[Category: Roesch P]]
-
[[Category: hormone]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:21:04 2007''
+

Current revision

STRUCTURE OF HUMAN PARATHYROID HORMONE 1-37 IN SOLUTION

PDB ID 1hph

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hph"
Personal tools