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| - | [[Image:1j8g.gif|left|200px]] | |
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| - | <!--
| + | ==X-ray Analysis of a RNA Tetraplex r(uggggu)4 at Ultra-High Resolution== |
| - | The line below this paragraph, containing "STRUCTURE_1j8g", creates the "Structure Box" on the page.
| + | <StructureSection load='1j8g' size='340' side='right'caption='[[1j8g]], [[Resolution|resolution]] 0.61Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1j8g]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J8G FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.61Å</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr> |
| - | {{STRUCTURE_1j8g| PDB=1j8g | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j8g OCA], [https://pdbe.org/1j8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j8g RCSB], [https://www.ebi.ac.uk/pdbsum/1j8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j8g ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''X-ray Analysis of a RNA Tetraplex r(uggggu)4 at Ultra-High Resolution'''
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | ==Overview== | + | [[Category: Deng J]] |
| - | Four-stranded guanine tetraplexes in RNA have been identified to be involved in crucial biological functions, such as dimerization of retroviral RNA, translational repression, and mRNA turnover. However, the structural basis for these biological processes is still largely unknown. Here we report the RNA tetraplex structure (UGGGGU)(4) at ultra-high resolution (0.61 A). The space group is P42(1)2, and cell constants are a = b = 36.16 A and c = 74.09 A. The structure was solved by the multiple-wavelength anomalous dispersion method using a set of three-wavelength data of the isomorphous bromo derivative (br)UGGGGU and refined to 0.61-A resolution. Each of the four strands in the asymmetric unit forms a parallel tetraplex with symmetry-related molecules. The tetraplex molecules stack on one another in opposite polarity (head-to-head or tail-to-tail) to form a pseudocontinuous column. All of the 5'-end uridines rotate around the backbone of G2, swing out, and form unique octaplexes with the neighboring G tetraplexes, whereas the 3'-end uridines are stacked-in and form uridine tetrads. All of the bases are anti, and the riboses are in the mixed C2'- and C3'-puckering mode. Strontium ions are observed in every other guanine tetrad plane, sitting on the fourfold axis and associated to the eight O6 atoms of neighboring guanine bases in a bipyramidal-antiprism geometry. The hydrogens are clearly observed in the structure.
| + | [[Category: Sundaralingam M]] |
| - | | + | [[Category: Xiong Y]] |
| - | ==About this Structure== | + | |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8G OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | X-ray analysis of an RNA tetraplex (UGGGGU)(4) with divalent Sr(2+) ions at subatomic resolution (0.61 A)., Deng J, Xiong Y, Sundaralingam M, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13665-70. Epub 2001 Nov 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11707581 11707581] | + | |
| - | [[Category: Deng, J.]] | + | |
| - | [[Category: Sundaralingam, M.]] | + | |
| - | [[Category: Xiong, Y.]] | + | |
| - | [[Category: Octaplex]] | + | |
| - | [[Category: Rna tetraplex]]
| + | |
| - | [[Category: Sr ion]] | + | |
| - | [[Category: U tetrad]] | + | |
| - | [[Category: Ultra-high resolution]] | + | |
| - | [[Category: X-ray]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:54:57 2008''
| + | |
