1j8l

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[[Image:1j8l.gif|left|200px]]
 
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==Molecular and Crystal Structure of D(CGCAAATTMO4CGCG): the Watson-Crick Type N4-Methoxycytidine/Adenosine Base Pair in B-DNA==
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The line below this paragraph, containing "STRUCTURE_1j8l", creates the "Structure Box" on the page.
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<StructureSection load='1j8l' size='340' side='right'caption='[[1j8l]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1j8l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J8L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C45:N4-METHOXY-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>C45</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1j8l| PDB=1j8l | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j8l OCA], [https://pdbe.org/1j8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j8l RCSB], [https://www.ebi.ac.uk/pdbsum/1j8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j8l ProSAT]</span></td></tr>
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</table>
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'''Molecular and Crystal Structure of D(CGCAAATTMO4CGCG): the Watson-Crick Type N4-Methoxycytidine/Adenosine Base Pair in B-DNA'''
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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==Overview==
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To investigate the mutation mechanism of purine transitions in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCAAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and the crystal structure determined by X-ray analysis. The duplex structure is similar to that of the original undamaged B-form dodecamer, indicating that the methoxylation does not affect the overall DNA conformation. Electron density maps clearly show that the two mo(4)C residues form Watson-Crick-type base pairs with the adenine residues of the opposite strand and that the methoxy groups of mo(4)C adopt the anti conformation to N(3) around the C(4)-N(4) bond. For the pair formation through hydrogen bonds the mo(4)C residues are in the imino tautomeric state. Together with previous work, the present work establishes that the methoxylated cytosine residue can present two alternate faces for Watson-Crick base-pairing, thanks to the amino&lt;--&gt;imino tautomerism allowed by methoxylation. Based on this property, two gene transition routes are proposed.
To investigate the mutation mechanism of purine transitions in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCAAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and the crystal structure determined by X-ray analysis. The duplex structure is similar to that of the original undamaged B-form dodecamer, indicating that the methoxylation does not affect the overall DNA conformation. Electron density maps clearly show that the two mo(4)C residues form Watson-Crick-type base pairs with the adenine residues of the opposite strand and that the methoxy groups of mo(4)C adopt the anti conformation to N(3) around the C(4)-N(4) bond. For the pair formation through hydrogen bonds the mo(4)C residues are in the imino tautomeric state. Together with previous work, the present work establishes that the methoxylated cytosine residue can present two alternate faces for Watson-Crick base-pairing, thanks to the amino&lt;--&gt;imino tautomerism allowed by methoxylation. Based on this property, two gene transition routes are proposed.
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==About this Structure==
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Crystallographic studies on damaged DNAs IV. N( 4)-methoxycytosine shows a second face for Watson-Crick base-pairing, leading to purine transition mutagenesis.,Hossain MT, Sunami T, Tsunoda M, Hikima T, Chatake T, Ueno Y, Matsuda A, Takenaka A Nucleic Acids Res. 2001 Oct 1;29(19):3949-54. PMID:11574676<ref>PMID:11574676</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8L OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallographic studies on damaged DNAs IV. N( 4)-methoxycytosine shows a second face for Watson-Crick base-pairing, leading to purine transition mutagenesis., Hossain MT, Sunami T, Tsunoda M, Hikima T, Chatake T, Ueno Y, Matsuda A, Takenaka A, Nucleic Acids Res. 2001 Oct 1;29(19):3949-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11574676 11574676]
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</div>
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[[Category: Chatake, T.]]
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<div class="pdbe-citations 1j8l" style="background-color:#fffaf0;"></div>
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[[Category: Hikima, T.]]
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== References ==
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[[Category: Hossain, M T.]]
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<references/>
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[[Category: Matsuda, A.]]
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__TOC__
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[[Category: Sunami, T.]]
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</StructureSection>
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[[Category: Takenaka, A.]]
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[[Category: Large Structures]]
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[[Category: Tsunoda, M.]]
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[[Category: Chatake T]]
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[[Category: Ueno, Y.]]
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[[Category: Hikima T]]
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[[Category: B-dna]]
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[[Category: Hossain MT]]
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[[Category: Deoxyribonucleic acid]]
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[[Category: Matsuda A]]
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[[Category: Dna damage]]
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[[Category: Sunami T]]
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[[Category: Double helix]]
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[[Category: Takenaka A]]
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[[Category: N4-methoxycytosine]]
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[[Category: Tsunoda M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:55:16 2008''
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[[Category: Ueno Y]]

Current revision

Molecular and Crystal Structure of D(CGCAAATTMO4CGCG): the Watson-Crick Type N4-Methoxycytidine/Adenosine Base Pair in B-DNA

PDB ID 1j8l

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