1j8m

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[[Image:1j8m.jpg|left|200px]]
 
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==Signal Recognition Particle conserved GTPase domain from A. ambivalens==
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The line below this paragraph, containing "STRUCTURE_1j8m", creates the "Structure Box" on the page.
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<StructureSection load='1j8m' size='340' side='right'caption='[[1j8m]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1j8m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidianus_ambivalens Acidianus ambivalens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J8M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j8m OCA], [https://pdbe.org/1j8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j8m RCSB], [https://www.ebi.ac.uk/pdbsum/1j8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j8m ProSAT]</span></td></tr>
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{{STRUCTURE_1j8m| PDB=1j8m | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SRP54_ACIAM SRP54_ACIAM] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/1j8m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j8m ConSurf].
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<div style="clear:both"></div>
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'''Signal Recognition Particle conserved GTPase domain from A. ambivalens'''
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==See Also==
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*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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BACKGROUND: Protein targeting to the endoplasmic reticulum in eukaryotes and to the cell membrane in prokaryotes is mediated by the signal recognition particle (SRP) and its receptor (SR). Both contain conserved GTPase domains in the signal-peptide-binding proteins (SRP54 and Ffh) and the SR proteins (SRalpha and FtsY). These GTPases are involved in the regulation of protein targeting. Most studies so far have focussed on the SRP machinery of mammals and bacteria, leaving the SRP system of archaea less well understood. RESULTS: We report the crystal structure of the conserved GTPase (NG-Ffh) from the thermophilic archaeon Acidianus ambivalens at 2.0 A resolution and of the Thr112--&gt;Ala mutant, which is inactive in GTP hydrolysis. This is the first structure of an SRP component from an archaeon and allows for a detailed comparison with related structures from Escherichia coli and thermophilic bacteria. In particular, differences in the conserved consensus regions for nucleotide binding and the subdomain interfaces are observed, which provide information about the regulation of the GTPase. These interactions allow us to propose a common signalling mechanism for the SRP-SR system. CONCLUSIONS: The overall structure of SRP-GTPases is well conserved between bacteria and archaea, which indicates strong similarities in the regulation of the SRP-targeting pathway. Surprisingly, structure comparisons identified a homodimeric ATP-binding protein as the closest relative. A heterodimer model for the SRP-SR interaction is presented.
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==About this Structure==
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1J8M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acidianus_ambivalens Acidianus ambivalens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8M OCA].
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==Reference==
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The crystal structure of the conserved GTPase of SRP54 from the archaeon Acidianus ambivalens and its comparison with related structures suggests a model for the SRP-SRP receptor complex., Montoya G, Kaat K, Moll R, Schafer G, Sinning I, Structure. 2000 May 15;8(5):515-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10801496 10801496]
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[[Category: Acidianus ambivalens]]
[[Category: Acidianus ambivalens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kaat, K te.]]
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[[Category: Moll R]]
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[[Category: Moll, R.]]
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[[Category: Montoya G]]
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[[Category: Montoya, G.]]
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[[Category: Schafer G]]
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[[Category: Schafer, G.]]
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[[Category: Sinning I]]
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[[Category: Sinning, I.]]
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[[Category: Te Kaat K]]
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[[Category: Signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:55:16 2008''
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Current revision

Signal Recognition Particle conserved GTPase domain from A. ambivalens

PDB ID 1j8m

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