1j95

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[[Image:1j95.jpg|left|200px]]
 
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==KCSA potassium channel with TBA (tetrabutylammonium) and potassium==
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The line below this paragraph, containing "STRUCTURE_1j95", creates the "Structure Box" on the page.
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<StructureSection load='1j95' size='340' side='right'caption='[[1j95]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1j95]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J95 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=TBA:TETRABUTYLAMMONIUM+ION'>TBA</scene></td></tr>
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{{STRUCTURE_1j95| PDB=1j95 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j95 OCA], [https://pdbe.org/1j95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j95 RCSB], [https://www.ebi.ac.uk/pdbsum/1j95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j95 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/1j95_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j95 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many voltage-dependent K+ channels open when the membrane is depolarized and then rapidly close by a process called inactivation. Neurons use inactivating K+ channels to modulate their firing frequency. In Shaker-type K+ channels, the inactivation gate, which is responsible for the closing of the channel, is formed by the channel's cytoplasmic amino terminus. Here we show that the central cavity and inner pore of the K+ channel form the receptor site for both the inactivation gate and small-molecule inhibitors. We propose that inactivation occurs by a sequential reaction in which the gate binds initially to the cytoplasmic channel surface and then enters the pore as an extended peptide. This mechanism accounts for the functional properties of K+ channel inactivation and indicates that the cavity may be the site of action for certain drugs that alter cation channel function.
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'''KCSA potassium channel with TBA (tetrabutylammonium) and potassium'''
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Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.,Zhou M, Morais-Cabral JH, Mann S, MacKinnon R Nature. 2001 Jun 7;411(6838):657-61. PMID:11395760<ref>PMID:11395760</ref>
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==Overview==
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Many voltage-dependent K+ channels open when the membrane is depolarized and then rapidly close by a process called inactivation. Neurons use inactivating K+ channels to modulate their firing frequency. In Shaker-type K+ channels, the inactivation gate, which is responsible for the closing of the channel, is formed by the channel's cytoplasmic amino terminus. Here we show that the central cavity and inner pore of the K+ channel form the receptor site for both the inactivation gate and small-molecule inhibitors. We propose that inactivation occurs by a sequential reaction in which the gate binds initially to the cytoplasmic channel surface and then enters the pore as an extended peptide. This mechanism accounts for the functional properties of K+ channel inactivation and indicates that the cavity may be the site of action for certain drugs that alter cation channel function.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1J95 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J95 OCA].
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</div>
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<div class="pdbe-citations 1j95" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors., Zhou M, Morais-Cabral JH, Mann S, MacKinnon R, Nature. 2001 Jun 7;411(6838):657-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11395760 11395760]
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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[[Category: Single protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces lividans]]
[[Category: Streptomyces lividans]]
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[[Category: MacKinnon, R.]]
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[[Category: MacKinnon R]]
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[[Category: Morais-Cabral, J H.]]
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[[Category: Morais-Cabral JH]]
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[[Category: Zhou, M.]]
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[[Category: Zhou M]]
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[[Category: Membrane protein]]
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[[Category: Metal transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:56:12 2008''
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Current revision

KCSA potassium channel with TBA (tetrabutylammonium) and potassium

PDB ID 1j95

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