This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7bb5
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7bb5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans_serotype_e_str._SC1083 Aggregatibacter actinomycetemcomitans serotype e str. SC1083]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7bb5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans_serotype_e_str._SC1083 Aggregatibacter actinomycetemcomitans serotype e str. SC1083]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/G4A6K5_AGGAC G4A6K5_AGGAC] | [https://www.uniprot.org/uniprot/G4A6K5_AGGAC G4A6K5_AGGAC] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | CRISPR-Cas systems are prokaryotic adaptive immune systems that protect against phages and other invading nucleic acids. The evolutionary arms race between prokaryotes and phages gave rise to phage anti-CRISPR (Acr) proteins that act as a counter defence against CRISPR-Cas systems by inhibiting the effector complex. Here, we used a combination of bulk biochemical experiments, X-ray crystallography and single-molecule techniques to explore the inhibitory activity of AcrIF6 and AcrIF9 proteins against the type I-F CRISPR-Cas system from Aggregatibacter actinomycetemcomitans (Aa). We showed that AcrIF6 and AcrIF9 proteins hinder Aa-Cascade complex binding to target DNA. We solved a crystal structure of Aa1-AcrIF9 protein, which differ from other known AcrIF9 proteins by an additional structurally important loop presumably involved in the interaction with Cascade. We revealed that AcrIF9 association with Aa-Cascade promotes its binding to off-target DNA sites, which facilitates inhibition of CRISPR-Cas protection. | ||
| - | |||
| - | Disarming of type I-F CRISPR-Cas surveillance complex by anti-CRISPR proteins AcrIF6 and AcrIF9.,Kupcinskaite E, Tutkus M, Kopustas A, Asmontas S, Jankunec M, Zaremba M, Tamulaitiene G, Sinkunas T Sci Rep. 2022 Sep 15;12(1):15548. doi: 10.1038/s41598-022-19797-y. PMID:36109551<ref>PMID:36109551</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 7bb5" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of anti-CRISPR protein AcrIF9
| |||||||||||
