1jb1

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[[Image:1jb1.gif|left|200px]]
 
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==Lactobacillus casei HprK/P Bound to Phosphate==
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The line below this paragraph, containing "STRUCTURE_1jb1", creates the "Structure Box" on the page.
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<StructureSection load='1jb1' size='340' side='right'caption='[[1jb1]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jb1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacticaseibacillus_casei Lacticaseibacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JB1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1jb1| PDB=1jb1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jb1 OCA], [https://pdbe.org/1jb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jb1 RCSB], [https://www.ebi.ac.uk/pdbsum/1jb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jb1 ProSAT]</span></td></tr>
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</table>
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'''Lactobacillus casei HprK/P Bound to Phosphate'''
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== Function ==
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[https://www.uniprot.org/uniprot/HPRK_LACCA HPRK_LACCA] Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of 'Ser-46' in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.[HAMAP-Rule:MF_01249]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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HPr kinase/phosphatase (HprK/P) is a key regulatory enzyme controlling carbon metabolism in Gram- positive bacteria. It catalyses the ATP-dependent phosphorylation of Ser46 in HPr, a protein of the phosphotransferase system, and also its dephosphorylation. HprK/P is unrelated to eukaryotic protein kinases, but contains the Walker motif A characteristic of nucleotide-binding proteins. We report here the X-ray structure of an active fragment of Lactobacillus casei HprK/P at 2.8 A resolution, solved by the multiwavelength anomalous dispersion method on a seleniated protein (PDB code 1jb1). The protein is a hexamer, with each subunit containing an ATP-binding domain similar to nucleoside/nucleotide kinases, and a putative HPr-binding domain unrelated to the substrate-binding domains of other kinases. The Walker motif A forms a typical P-loop which binds inorganic phosphate in the crystal. We modelled ATP binding by comparison with adenylate kinase, and designed a tentative model of the complex with HPr based on a docking simulation. The results confirm that HprK/P represents a new family of protein kinases, first identified in bacteria, but which may also have members in eukaryotes.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/1jb1_consurf.spt"</scriptWhenChecked>
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1JB1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JB1 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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X-ray structure of HPr kinase: a bacterial protein kinase with a P-loop nucleotide-binding domain., Fieulaine S, Morera S, Poncet S, Monedero V, Gueguen-Chaignon V, Galinier A, Janin J, Deutscher J, Nessler S, EMBO J. 2001 Aug 1;20(15):3917-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11483495 11483495]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jb1 ConSurf].
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[[Category: Lactobacillus casei]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Deutscher, J.]]
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</StructureSection>
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[[Category: Fieulaine, S.]]
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[[Category: Lacticaseibacillus casei]]
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[[Category: Galinier, A.]]
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[[Category: Large Structures]]
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[[Category: Gueguen-Chaignon, V.]]
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[[Category: Deutscher J]]
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[[Category: Janin, J.]]
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[[Category: Fieulaine S]]
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[[Category: Monedero, V.]]
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[[Category: Galinier A]]
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[[Category: Morera, S.]]
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[[Category: Gueguen-Chaignon V]]
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[[Category: Nessler, S.]]
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[[Category: Janin J]]
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[[Category: Poncet, S.]]
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[[Category: Monedero V]]
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[[Category: Catabolite repression]]
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[[Category: Morera S]]
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[[Category: Hexamer]]
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[[Category: Nessler S]]
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[[Category: Hpr phosphorylation]]
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[[Category: Poncet S]]
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[[Category: Lactobacillus casei]]
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[[Category: P-loop]]
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[[Category: Protein kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:00:04 2008''
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Current revision

Lactobacillus casei HprK/P Bound to Phosphate

PDB ID 1jb1

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