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| - | [[Image:1jb8.gif|left|200px]] | |
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| - | <!--
| + | ==The Crystal Structure of an RNA/DNA Hybrid Reveals Novel Intermolecular Intercalation== |
| - | The line below this paragraph, containing "STRUCTURE_1jb8", creates the "Structure Box" on the page.
| + | <StructureSection load='1jb8' size='340' side='right'caption='[[1jb8]], [[Resolution|resolution]] 2.38Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1jb8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JB8 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jb8 OCA], [https://pdbe.org/1jb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jb8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jb8 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1jb8| PDB=1jb8 | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''The Crystal Structure of an RNA/DNA Hybrid Reveals Novel Intermolecular Intercalation'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Dickerson RE]] |
| - | ==Overview== | + | [[Category: Han GW]] |
| - | An intermolecular intercalation of base pairs was found at the CA step in the I222 crystal structure of the RNA.DNA hybrid, r(CAAAGAAAAG).d(CTTTTCTTTG), which contains two-thirds of the polypurine tract sequence of HIV-1 with a substitution of cytosine for the initial adenine. This sequence crystallized in both P212121 and I222 space groups, with an rms difference of only 0.63 A between residues 3 to 18 of the two forms. P212121 and I222 helices are both A-like, but intercalation occurs only in the I222 crystal form. The present structure shows bases stacked in parallel rather than perpendicular as in intercalated DNA (I-DNA). The base intercalation is also different from zipper-like meshing of bases seen in the center of the crystal structure of d(GCGAAAGCT), which does not have Watson-Crick base pairing. The base-step intercalation seen here is reminiscent of domain swapping in proteins; therefore, we call this phenomenon "base-pair swapping." It involves a highly mobile CA step and seems to be sequence-specific and electrostatically stable without disrupting Watson-Crick interactions. It also exhibits a large rise concurrent with unwinding of the helix (low twist). We present a base-pair swapping dimer in nucleic acids.
| + | [[Category: Kopka ML]] |
| - | | + | [[Category: Langs D]] |
| - | ==About this Structure== | + | |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JB8 OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Crystal structure of an RNA.DNA hybrid reveals intermolecular intercalation: dimer formation by base-pair swapping., Han GW, Kopka ML, Langs D, Sawaya MR, Dickerson RE, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9214-9. Epub 2003 Jul 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12872000 12872000]
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| - | [[Category: Dickerson, R E.]] | + | |
| - | [[Category: Han, G W.]] | + | |
| - | [[Category: Kopka, M L.]] | + | |
| - | [[Category: Langs, D.]] | + | |
| - | [[Category: Dna/dna intercalation]] | + | |
| - | [[Category: Rna/dna hybrid]] | + | |
| - | [[Category: Rna/dna polypurine tract]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:00:29 2008''
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