1ev7
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1ev7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EV7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1ev7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EV7 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [https://pdbe.org/1ev7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ev7 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [https://pdbe.org/1ev7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ev7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref> | [https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes. | ||
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| - | Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase.,Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254<ref>PMID:10856254</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1ev7" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
Current revision
CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI
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