This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1i1b

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1i1b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i1b, resolution 2.0&Aring;" /> '''CRYSTAL STRUCTURE OF...)
Current revision (07:31, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1i1b.gif|left|200px]]<br />
 
-
<applet load="1i1b" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1i1b, resolution 2.0&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-1BETA AT 2.0 ANGSTROMS RESOLUTION'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-1BETA AT 2.0 ANGSTROMS RESOLUTION==
-
The crystal structure of recombinant human interleukin-1 beta (IL-1 beta), has been determined at 2.0 A resolution and refined to a crystallographic, R-factor of 0.19. Three heavy-atom derivatives were identified and used, for multiple isomorphous replacement phasing. Interpretation of the, resulting electron density map revealed a structure in which there are 12, antiparallel beta-strands and no alpha-helix. The single 153-residue, polypeptide chain is folded into a six-stranded beta-barrel similar in, architecture to the Kunitz-type trypsin inhibitor found in soybeans. The, molecule displays approximate 3-fold symmetry about the axis of the, beta-barrel. Each successive pair of component strands of the barrel, brackets an extensive sequence outside the barrel that includes an, additional pair of beta-strands and a prominent loop. Together, these, three external segments conceal much of the perimeter and one end of the, barrel, leaving only the end supporting the chain termini fully exposed., The structure can be used to identify portions of the polypeptide chain, that are exposed on the surface of the molecule, some of which must be, epitopes recognized by interleukin-1 beta receptors.
+
<StructureSection load='1i1b' size='340' side='right'caption='[[1i1b]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1i1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I1B FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i1b OCA], [https://pdbe.org/1i1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i1b RCSB], [https://www.ebi.ac.uk/pdbsum/1i1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i1b ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/1i1b_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i1b ConSurf].
 +
<div style="clear:both"></div>
-
==Disease==
+
==See Also==
-
Known disease associated with this structure: Gastric cancer risk after H. pylori infection OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147720 147720]]
+
*[[Interleukin 3D structures|Interleukin 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1I1B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I1B OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
Crystal structure of recombinant human interleukin-1 beta at 2.0 A resolution., Finzel BC, Clancy LL, Holland DR, Muchmore SW, Watenpaugh KD, Einspahr HM, J Mol Biol. 1989 Oct 20;209(4):779-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2585509 2585509]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Einspahr, H.M.]]
+
[[Category: Einspahr HM]]
-
[[Category: Finzel, B.C.]]
+
[[Category: Finzel BC]]
-
[[Category: Watenpaugh, K.D.]]
+
[[Category: Watenpaugh KD]]
-
[[Category: cytokine]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:25:09 2007''
+

Current revision

CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-1BETA AT 2.0 ANGSTROMS RESOLUTION

PDB ID 1i1b

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i1b"
Personal tools