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1jif

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[[Image:1jif.gif|left|200px]]
 
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==Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin==
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The line below this paragraph, containing "STRUCTURE_1jif", creates the "Structure Box" on the page.
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<StructureSection load='1jif' size='340' side='right'caption='[[1jif]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jif]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JIF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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{{STRUCTURE_1jif| PDB=1jif | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jif OCA], [https://pdbe.org/1jif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jif RCSB], [https://www.ebi.ac.uk/pdbsum/1jif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jif ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q53793_9ACTN Q53793_9ACTN]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Bleomycin (Bm) in the culture broth of Streptomyces verticillus is complexed with Cu(2+) (Cu(II)). In the present study, we determined the x-ray crystal structures of the Cu(II)-bound and the metal-free types of Bm at a high resolution of 1.6 and 1.8 A, respectively, which are complexed with a Bm resistance determinant from Bm-producing S. verticillus, designated BLMA. In the current model of Cu(II).Bm complexed with BLMA, two Cu(II).Bm molecules bind to the BLMA dimer. The electron density map shows that the copper ion is clearly defined in the metal-binding domain of the Bm molecule. The metal ion is penta-coordinated by a tetragonal monopyramidal cage of nitrogens and binds to the primary amine of the beta-aminoalanine moiety of Bm. The binding experiment between Bm and BLMA showed that each of the two Bm-binding pockets has a different dissociation constant (K(d)(1) and K(d)(2)). The K(d)(1) value of 630 nm for the first Bm binding is larger than the K(d)(2) value of 120 nm, indicating that the first Bm binding gives rise to a cooperative binding of the second Bm to the other pocket.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1jif_consurf.spt"</scriptWhenChecked>
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1JIF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JIF OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The 1.6-A crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus., Sugiyama M, Kumagai T, Hayashida M, Maruyama M, Matoba Y, J Biol Chem. 2002 Jan 18;277(3):2311-20. Epub 2001 Nov 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11706014 11706014]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jif ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces verticillus]]
[[Category: Streptomyces verticillus]]
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[[Category: Hayashida, M.]]
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[[Category: Hayashida M]]
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[[Category: Kumagai, T.]]
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[[Category: Kumagai T]]
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[[Category: Maruyama, M.]]
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[[Category: Maruyama M]]
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[[Category: Matoba, Y.]]
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[[Category: Matoba Y]]
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[[Category: Sugiyama, M.]]
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[[Category: Sugiyama M]]
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[[Category: Protein-ligand complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:15:45 2008''
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Current revision

Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin

PDB ID 1jif

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