1i49

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(New page: 200px<br /> <applet load="1i49" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i49, resolution 2.8&Aring;" /> '''CRYSTAL STRUCTURE AN...)
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[[Image:1i49.gif|left|200px]]<br />
 
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<applet load="1i49" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i49, resolution 2.8&Aring;" />
 
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'''CRYSTAL STRUCTURE ANALYSIS OF ARFAPTIN'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE ANALYSIS OF ARFAPTIN==
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Small G proteins are GTP-dependent molecular switches that regulate, numerous cellular functions. They can be classified into homologous, subfamilies that are broadly associated with specific biological, processes. Cross-talk between small G-protein families has an important, role in signalling, but the mechanism by which it occurs is poorly, understood. The coordinated action of Arf and Rho family GTPases is, required to regulate many cellular processes including lipid signalling, cell motility and Golgi function. Arfaptin is a ubiquitously expressed, protein implicated in mediating cross-talk between Rac (a member of the, Rho family) and Arf small GTPases. Here we show that Arfaptin binds, specifically to GTP-bound Arf1 and Arf6, but binds to Rac.GTP and Rac.GDP, with similar affinities. The X-ray structure of Arfaptin reveals an, elongated, crescent-shaped dimer of three-helix coiled-coils. Structures, of Arfaptin with Rac bound to either GDP or the slowly hydrolysable, analogue GMPPNP show that the switch regions adopt similar conformations, in both complexes. Our data highlight fundamental differences between the, molecular mechanisms of Rho and Ras family signalling, and suggest a model, of Arfaptin-mediated synergy between the Arf and Rho family signalling, pathways.
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<StructureSection load='1i49' size='340' side='right'caption='[[1i49]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i49]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I49 FirstGlance]. <br>
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1I49 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I49 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i49 OCA], [https://pdbe.org/1i49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i49 RCSB], [https://www.ebi.ac.uk/pdbsum/1i49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i49 ProSAT]</span></td></tr>
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==Reference==
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</table>
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The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways., Tarricone C, Xiao B, Justin N, Walker PA, Rittinger K, Gamblin SJ, Smerdon SJ, Nature. 2001 May 10;411(6834):215-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11346801 11346801]
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== Function ==
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[https://www.uniprot.org/uniprot/ARFP2_HUMAN ARFP2_HUMAN] Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/1i49_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i49 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gamblin, S.J.]]
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[[Category: Gamblin SJ]]
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[[Category: Justin, N.]]
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[[Category: Justin N]]
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[[Category: Smerdon, S.J.]]
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[[Category: Smerdon SJ]]
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[[Category: Tarricone, C.]]
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[[Category: Tarricone C]]
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[[Category: Xiao, B.]]
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[[Category: Xiao B]]
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[[Category: signaling protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:26:02 2007''
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Current revision

CRYSTAL STRUCTURE ANALYSIS OF ARFAPTIN

PDB ID 1i49

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