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| - | [[Image:1jjd.gif|left|200px]] | |
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| - | <!--
| + | ==NMR structure of the Cyanobacterial Metallothionein SmtA== |
| - | The line below this paragraph, containing "STRUCTURE_1jjd", creates the "Structure Box" on the page.
| + | <StructureSection load='1jjd' size='340' side='right'caption='[[1jjd]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1jjd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJD FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
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| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | {{STRUCTURE_1jjd| PDB=1jjd | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjd OCA], [https://pdbe.org/1jjd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jjd RCSB], [https://www.ebi.ac.uk/pdbsum/1jjd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjd ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''NMR structure of the Cyanobacterial Metallothionein SmtA'''
| + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/MT_SYNE7 MT_SYNE7] May play a role in essential metal ion homeostasis (especially zinc homeostasis) and resistance to certain non-essential metal ions. Metallothioneins have a high content of cysteine residues that bind various heavy metals. |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | Zinc is essential for many cellular processes, including DNA synthesis, transcription, and translation, but excess can be toxic. A zinc-induced gene, smtA, is required for normal zinc-tolerance in the cyanobacterium Synechococcus PCC 7942. Here we report that the protein SmtA contains a cleft lined with Cys-sulfur and His-imidazole ligands that binds four zinc ions in a Zn(4)Cys(9)His(2) cluster. The thiolate sulfurs of five Cys ligands provide bridges between the two ZnCys(4) and two ZnCys(3)His sites, giving two fused six-membered rings with distorted boat conformations. The inorganic core strongly resembles the Zn(4)Cys(11) cluster of mammalian metallothionein, despite different amino acid sequences, a different linear order of the ligands, and presence of histidine ligands. Also, SmtA contains elements of secondary structure not found in metallothioneins. One of the two Cys(4)-coordinated zinc ions in SmtA readily exchanges with exogenous metal ((111)Cd), whereas the other is inert. The thiolate sulfur ligands bound to zinc in this site are buried within the protein. Regions of beta-strand and alpha-helix surround the inert site to form a zinc finger resembling the zinc fingers in GATA and LIM-domain proteins. Eukaryotic zinc fingers interact specifically with other proteins or DNA and an analogous interaction can therefore be anticipated for prokaryotic zinc fingers. SmtA now provides structural proof for the existence of zinc fingers in prokaryotes, and sequences related to the zinc finger motif can be identified in several bacterial genomes.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Synechococcus elongatus PCC 7942 = FACHB-805]] |
| - | ==About this Structure== | + | [[Category: Robinson NJ]] |
| - | 1JJD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJD OCA].
| + | [[Category: Sadler PJ]] |
| - | | + | |
| - | ==Reference== | + | |
| - | A metallothionein containing a zinc finger within a four-metal cluster protects a bacterium from zinc toxicity., Blindauer CA, Harrison MD, Parkinson JA, Robinson AK, Cavet JS, Robinson NJ, Sadler PJ, Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9593-8. Epub 2001 Aug 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11493688 11493688]
| + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Synechococcus sp.]]
| + | |
| - | [[Category: Robinson, N J.]] | + | |
| - | [[Category: Sadler, P J.]] | + | |
| - | [[Category: Metallothionein]] | + | |
| - | [[Category: Zinc cluster]] | + | |
| - | [[Category: Zinc finger]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:17:42 2008''
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