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1jmt

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[[Image:1jmt.jpg|left|200px]]
 
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==X-ray Structure of a Core U2AF65/U2AF35 Heterodimer==
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The line below this paragraph, containing "STRUCTURE_1jmt", creates the "Structure Box" on the page.
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<StructureSection load='1jmt' size='340' side='right'caption='[[1jmt]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jmt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JMT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>
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{{STRUCTURE_1jmt| PDB=1jmt | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jmt OCA], [https://pdbe.org/1jmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jmt RCSB], [https://www.ebi.ac.uk/pdbsum/1jmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jmt ProSAT]</span></td></tr>
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</table>
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'''X-ray Structure of a Core U2AF65/U2AF35 Heterodimer'''
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== Function ==
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[https://www.uniprot.org/uniprot/U2AF1_HUMAN U2AF1_HUMAN] Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.<ref>PMID:8647433</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes the 3' splice site and recruits the U2 snRNP to the branch point. The X-ray structure of the human core U2AF heterodimer, consisting of the U2AF35 central domain and a proline-rich region of U2AF65, has been determined at 2.2 A resolution. The structure reveals a novel protein-protein recognition strategy, in which an atypical RNA recognition motif (RRM) of U2AF35 and the U2AF65 polyproline segment interact via reciprocal "tongue-in-groove" tryptophan residues. Complementary biochemical experiments demonstrate that the core U2AF heterodimer binds RNA, and that the interacting tryptophan side chains are essential for U2AF dimerization. Atypical RRMs in other splicing factors may serve as protein-protein interaction motifs elsewhere during spliceosome assembly.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/1jmt_consurf.spt"</scriptWhenChecked>
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1JMT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JMT OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer., Kielkopf CL, Rodionova NA, Green MR, Burley SK, Cell. 2001 Sep 7;106(5):595-605. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11551507 11551507]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jmt ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Burley, S K.]]
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[[Category: Burley SK]]
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[[Category: Green, M R.]]
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[[Category: Green MR]]
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[[Category: Kielkopf, C L.]]
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[[Category: Kielkopf CL]]
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[[Category: Rodionova, N A.]]
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[[Category: Rodionova NA]]
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[[Category: Peptide recognition]]
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[[Category: Ppii helix]]
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[[Category: Proline]]
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[[Category: Rna splicing]]
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[[Category: Rrm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:25:41 2008''
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Current revision

X-ray Structure of a Core U2AF65/U2AF35 Heterodimer

PDB ID 1jmt

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