1jof

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[[Image:1jof.jpg|left|200px]]
 
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==Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme==
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The line below this paragraph, containing "STRUCTURE_1jof", creates the "Structure Box" on the page.
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<StructureSection load='1jof' size='340' side='right'caption='[[1jof]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jof]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JOF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PIN:PIPERAZINE-N,N-BIS(2-ETHANESULFONIC+ACID)'>PIN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1jof| PDB=1jof | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jof OCA], [https://pdbe.org/1jof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jof RCSB], [https://www.ebi.ac.uk/pdbsum/1jof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jof ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CMLE_NEUCR CMLE_NEUCR] Catalyzes a syn cycloisomerization. Also possesses mle activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/1jof_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jof ConSurf].
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<div style="clear:both"></div>
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'''Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme'''
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==See Also==
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*[[Muconate cycloisomerase|Muconate cycloisomerase]]
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__TOC__
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==Overview==
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</StructureSection>
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Muconate lactonizing enzymes (MLEs) convert cis,cis-muconates to muconolactones in microbes as part of the beta-ketoadipate pathway; some also dehalogenate muconate derivatives of xenobiotic haloaromatics. There are three different MLE classes unrelated by evolution. We present the X-ray structure of a eukaryotic MLE, Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme (NcCMLE) at 2.5 A resolution, with a seven-bladed beta propeller fold. It is related neither to bacterial MLEs nor to other beta propeller enzymes, but is structurally similar to the G protein beta subunit. It reveals a novel metal-independent cycloisomerase motif unlike the bacterial metal cofactor MLEs. Together, the bacterial MLEs and NcCMLE structures comprise a striking structural example of functional convergence in enzymes for 1,2-addition-elimination of carboxylic acids. NcCMLE and bacterial MLEs may enhance the reaction rate differently: the former by electrophilic catalysis and the latter by electrostatic stabilization of the enolate.
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[[Category: Large Structures]]
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==About this Structure==
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1JOF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOF OCA].
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==Reference==
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The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase., Kajander T, Merckel MC, Thompson A, Deacon AM, Mazur P, Kozarich JW, Goldman A, Structure. 2002 Apr;10(4):483-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11937053 11937053]
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[[Category: Carboxy-cis,cis-muconate cyclase]]
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[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
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[[Category: Single protein]]
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[[Category: Deacon AM]]
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[[Category: Deacon, A M.]]
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[[Category: Goldman A]]
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[[Category: Goldman, A.]]
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[[Category: Kajander T]]
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[[Category: Kajander, T.]]
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[[Category: Kozarich JW]]
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[[Category: Kozarich, J W.]]
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[[Category: Mazur P]]
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[[Category: Mazur, P.]]
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[[Category: Merckel MC]]
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[[Category: Merckel, M C.]]
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[[Category: Thompson A]]
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[[Category: Thompson, A.]]
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[[Category: Beta-propeller]]
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[[Category: Homotetramer]]
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[[Category: Semet-protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:30:12 2008''
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Current revision

Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme

PDB ID 1jof

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