8hx3
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==crystal structure of secreted coleopteran active protein (Sip)== | |
| + | <StructureSection load='8hx3' size='340' side='right'caption='[[8hx3]], [[Resolution|resolution]] 2.28Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8hx3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HX3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.28Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hx3 OCA], [https://pdbe.org/8hx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hx3 RCSB], [https://www.ebi.ac.uk/pdbsum/8hx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hx3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A2Z0MQV7_BACTU A0A2Z0MQV7_BACTU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | BACKGROUND: Microbially derived, protein-based biopesticides are an important approach for sustainable pest management. The secreted insecticidal proteins (Sips) produced by the bacterium Bacillus thuringiensis exhibit potent insecticidal activity against coleopteran pests and are, therefore, attractive as candidate biopesticides. However, the modes-of-action of Sips are unclear as comprehensive structural information for these proteins is lacking. RESULTS: Using X-ray crystallography, we elucidated the structure of monomeric Sip1Ab at 2.28 A resolution. Structural analyses revealed that Sip1Ab has the three domains and conserved fold characteristic of other aerolysin-like beta-pore-forming toxins (beta-PFTs). Based on the sequence and structural similarities between Sip1Ab and other ETX_MTX2 subfamily toxins, we suggested the mechanism of these proteins and proposed that it is common to them all. CONCLUSION: The atomic-level structural data for Sip1Ab generated by the present study could facilitate future structural and mechanistic research on Sips as well as their application in sustainable insect pest management. (c) 2023 Society of Chemical Industry. | ||
| - | + | Structural insight into Bacillus thuringiensis Sip1Ab reveals its similarity to ETX_MTX2 family beta-pore-forming toxin.,Chen Z, Shi Y, Wang D, Liu X, Jiao X, Gao X, Jiang K Pest Manag Sci. 2023 Jun 21. doi: 10.1002/ps.7622. PMID:37341620<ref>PMID:37341620</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8hx3" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chen | + | == References == |
| - | [[Category: Jiang | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus thuringiensis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chen Z]] | ||
| + | [[Category: Gao X]] | ||
| + | [[Category: Jiang K]] | ||
Current revision
crystal structure of secreted coleopteran active protein (Sip)
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