1iei

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(New page: 200px<br /> <applet load="1iei" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iei, resolution 2.5&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1iei.gif|left|200px]]<br />
 
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<applet load="1iei" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1iei, resolution 2.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF HUMAN ALDOSE REDUCTASE COMPLEXED WITH THE INHIBITOR ZENARESTAT.'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF HUMAN ALDOSE REDUCTASE COMPLEXED WITH THE INHIBITOR ZENARESTAT.==
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The crystal structure of the complex of human recombinant aldose reductase, (AR) with zenarestat, one of its potent inhibitors, has been solved at 2.5, A resolution. Zenarestat fits neatly in the hydrophobic active site and, induces unique and dramatic conformational changes. For example, the, benzene ring of zenarestat occupies a gap in the side chains of Leu300 and, Trp111 that interact directly and forms a CH-pi interaction in the native, holoenzyme. As a result, the benzene ring of the inhibitor and these side, chains form a CH-pi-pi interaction. Such structural information is key to, understanding the mode of action of this class of inhibitors and for, rational design of better therapeutics.
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<StructureSection load='1iei' size='340' side='right'caption='[[1iei]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1iei]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IEI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=ZES:[3-(4-BROMO-2-FLUORO-BENZYL)-7-CHLORO-2,4-DIOXO-3,4-DIHYDRO-2H-QUINAZOLIN-1-YL]-ACETIC+ACID'>ZES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iei OCA], [https://pdbe.org/1iei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iei RCSB], [https://www.ebi.ac.uk/pdbsum/1iei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iei ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALDR_HUMAN ALDR_HUMAN] Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/1iei_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iei ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1IEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAP and ZES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aldehyde_reductase Aldehyde reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.21 1.1.1.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IEI OCA].
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*[[Aldose reductase 3D structures|Aldose reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The structure of human recombinant aldose reductase complexed with the potent inhibitor zenarestat., Kinoshita T, Miyake H, Fujii T, Takakura S, Goto T, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):622-6. Epub 2002, Mar 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11914486 11914486]
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[[Category: Aldehyde reductase]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fujii, T.]]
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[[Category: Fujii T]]
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[[Category: Goto, T.]]
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[[Category: Goto T]]
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[[Category: Kinoshita, T.]]
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[[Category: Kinoshita T]]
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[[Category: Miyake, H.]]
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[[Category: Miyake H]]
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[[Category: Takakura, S.]]
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[[Category: Takakura S]]
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[[Category: NAP]]
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[[Category: ZES]]
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[[Category: nadp]]
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[[Category: protein-inhibitor complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:29:08 2007''
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Current revision

CRYSTAL STRUCTURE OF HUMAN ALDOSE REDUCTASE COMPLEXED WITH THE INHIBITOR ZENARESTAT.

PDB ID 1iei

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