7zbd
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7zbd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZBD FirstGlance]. <br> | <table><tr><td colspan='2'>[[7zbd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZBD FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ILQ:( | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ILQ:(10~{R})-7-azanyl-~{N}-[2-[2-(6-chloranylhexoxy)ethoxy]ethyl]-2-cyano-5,5-dimethyl-3-(methylamino)-1-oxidanylidene-spiro[benzo[b][1]benzosiline-10,3-isoindole]-5-carboxamide'>ILQ</scene>, <scene name='pdbligand=ILU:[7-azanyl-10-[5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]-2-(cyanocarbamoyl)phenyl]-5,5-dimethyl-benzo[b][1]benzosilin-3-ylidene]-methyl-azanium'>ILU</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zbd OCA], [https://pdbe.org/7zbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zbd RCSB], [https://www.ebi.ac.uk/pdbsum/7zbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zbd ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zbd OCA], [https://pdbe.org/7zbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zbd RCSB], [https://www.ebi.ac.uk/pdbsum/7zbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zbd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/DHAA_RHOSO DHAA_RHOSO] | [https://www.uniprot.org/uniprot/DHAA_RHOSO DHAA_RHOSO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glutathione (GSH) is the main determinant of intracellular redox potential and participates in multiple cellular signalling pathways. Achieving a detailed understanding of intracellular GSH homeostasis depends on the development of tools to map GSH compartmentalization and intra-organelle fluctuations. Here we present a GSH-sensing platform for live-cell imaging, termed targetable ratiometric quantitative GSH (TRaQ-G). This chemogenetic sensor possesses a unique reactivity turn-on mechanism, ensuring that the small molecule is only sensitive to GSH in a desired location. Furthermore, TRaQ-G can be fused to a fluorescent protein to give a ratiometric response. Using TRaQ-G fused to a redox-insensitive fluorescent protein, we demonstrate that the nuclear and cytosolic GSH pools are independently regulated during cell proliferation. This sensor was used in combination with a redox-sensitive fluorescent protein to quantify redox potential and GSH concentration simultaneously in the endoplasmic reticulum. Finally, by exchanging the fluorescent protein, we created a near-infrared, targetable and quantitative GSH sensor. | ||
| + | |||
| + | A locally activatable sensor for robust quantification of organellar glutathione.,Emmert S, Quargnali G, Thallmair S, Rivera-Fuentes P Nat Chem. 2023 Oct;15(10):1415-1421. doi: 10.1038/s41557-023-01249-3. Epub 2023 , Jun 15. PMID:37322101<ref>PMID:37322101</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7zbd" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Rhodococcus sp]] | [[Category: Rhodococcus sp]] | ||
| - | [[Category: | + | [[Category: Emmert S]] |
[[Category: Lau K]] | [[Category: Lau K]] | ||
[[Category: Pojer F]] | [[Category: Pojer F]] | ||
[[Category: Rivera-Fuentes P]] | [[Category: Rivera-Fuentes P]] | ||
Current revision
HaloTag with TRaQ-G ligand
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