1js1

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[[Image:1js1.gif|left|200px]]
 
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==Crystal Structure of a new transcarbamylase from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution==
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The line below this paragraph, containing "STRUCTURE_1js1", creates the "Structure Box" on the page.
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<StructureSection load='1js1' size='340' side='right'caption='[[1js1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1js1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_fragilis Bacteroides fragilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JS1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1js1| PDB=1js1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1js1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1js1 OCA], [https://pdbe.org/1js1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1js1 RCSB], [https://www.ebi.ac.uk/pdbsum/1js1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1js1 ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of a new transcarbamylase from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution'''
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== Function ==
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[https://www.uniprot.org/uniprot/SOTC_BACTN SOTC_BACTN] Catalyzes the transfer of the carbamoyl group from carbamoyl phosphate to the delta-amino group of N(2)-succinyl-L-ornithine to produce N(2)-succinyl-L-citrulline. Is essential for arginine biosynthesis. Has no activity with either L-ornithine or L-aspartate as substrate. Has also no detectable AOTCase activity, being unable to convert N(2)-acetyl-L-ornithine to N(2)-acetyl-L-citrulline.[UniProtKB:E1WKT5]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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A transcarbamylase-like protein essential for arginine biosynthesis in the anaerobic bacterium Bacteroides fragilis has been purified and crystallized in space group P4(3)2(1)2 (a=b=153.4 A, c=94.8 A). The structure was solved using a single isomorphous replacement with anomalous scattering (SIRAS) and was refined at 2.0 A resolution to an R-factor of 20.6% (R-free=25.2%). The molecular model is trimeric and comprises 960 amino acid residues, two phosphate groups and 422 water molecules. The monomer has the consensus transcarbamylase fold with two structural domains linked by two long interdomain helices: the putative carbamoyl phosphate-binding domain and a binding domain for the second substrate. Each domain has a central parallel beta-sheet surrounded by alpha-helices and loops with alpha/beta topology. The putative carbamoyl phosphate-binding site is similar to those in ornithine transcarbamylases (OTCases) and aspartate transcarbamylases (ATCases); however, the second substrate-binding site is strikingly different. This site has several insertions and deletions, and residues critical to substrate binding and catalysis in other known transcarbamylases are not conserved. The three-dimensional structure and the fact that this protein is essential for arginine biosynthesis suggest strongly that it is a new member of the transcarbamylase family. A similar protein has been found in Xylella fastidiosa, a bacterium that infects grapes, citrus and other plants.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/1js1_consurf.spt"</scriptWhenChecked>
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1JS1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteroides_fragilis Bacteroides fragilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS1 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of a transcarbamylase-like protein from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution., Shi D, Gallegos R, DePonte J 3rd, Morizono H, Yu X, Allewell NM, Malamy M, Tuchman M, J Mol Biol. 2002 Jul 19;320(4):899-908. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12095263 12095263]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1js1 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacteroides fragilis]]
[[Category: Bacteroides fragilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Allewell, N M.]]
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[[Category: Allewell NM]]
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[[Category: Gallegos, R.]]
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[[Category: DePonte III J]]
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[[Category: III, J DePonte.]]
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[[Category: Gallegos R]]
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[[Category: Malamy, M.]]
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[[Category: Malamy M]]
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[[Category: Morizono, H.]]
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[[Category: Morizono H]]
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[[Category: Shi, D.]]
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[[Category: Shi D]]
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[[Category: Tuchman, M.]]
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[[Category: Tuchman M]]
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[[Category: Yu, X.]]
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[[Category: Yu X]]
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[[Category: Alpha/beta topology]]
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[[Category: Two domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:48:26 2008''
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Current revision

Crystal Structure of a new transcarbamylase from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution

PDB ID 1js1

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