1juh

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[[Image:1juh.jpg|left|200px]]
 
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==Crystal Structure of Quercetin 2,3-dioxygenase==
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The line below this paragraph, containing "STRUCTURE_1juh", creates the "Structure Box" on the page.
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<StructureSection load='1juh' size='340' side='right'caption='[[1juh]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1juh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_japonicus Aspergillus japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JUH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_1juh| PDB=1juh | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1juh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1juh OCA], [https://pdbe.org/1juh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1juh RCSB], [https://www.ebi.ac.uk/pdbsum/1juh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1juh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/QDOI_ASPJA QDOI_ASPJA] Performs the first step in the degradation of the flavonoid quercetin by a dioxygenase reaction. The enzyme catalyzes the cleavage of the O-heteroaromatic ring of the flavonol quercetin yielding the depside 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. This involves the remarkable dioxygenolytic cleavage of two carbon-carbon bonds.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/1juh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1juh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Quercetin 2,3-dioxygenase is a copper-containing enzyme that catalyzes the insertion of molecular oxygen into polyphenolic flavonols. Dioxygenation catalyzed by iron-containing enzymes has been studied extensively, but dioxygenases employing other metal cofactors are poorly understood. We determined the crystal structure of quercetin 2,3-dioxygenase at 1.6 A resolution. The enzyme forms homodimers, which are stabilized by an N-linked heptasaccharide at the dimer interface. The mononuclear type 2 copper center displays two distinct geometries: a distorted tetrahedral coordination, formed by His66, His68, His112, and a water molecule, and a distorted trigonal bipyramidal environment, which additionally comprises Glu73. Manual docking of the substrate quercetin into the active site showed that the different geometries of the copper site might be of catalytic importance.
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'''Crystal Structure of Quercetin 2,3-dioxygenase'''
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Crystal structure of the copper-containing quercetin 2,3-dioxygenase from Aspergillus japonicus.,Fusetti F, Schroter KH, Steiner RA, van Noort PI, Pijning T, Rozeboom HJ, Kalk KH, Egmond MR, Dijkstra BW Structure. 2002 Feb;10(2):259-68. PMID:11839311<ref>PMID:11839311</ref>
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==Overview==
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Quercetin 2,3-dioxygenase is a copper-containing enzyme that catalyzes the insertion of molecular oxygen into polyphenolic flavonols. Dioxygenation catalyzed by iron-containing enzymes has been studied extensively, but dioxygenases employing other metal cofactors are poorly understood. We determined the crystal structure of quercetin 2,3-dioxygenase at 1.6 A resolution. The enzyme forms homodimers, which are stabilized by an N-linked heptasaccharide at the dimer interface. The mononuclear type 2 copper center displays two distinct geometries: a distorted tetrahedral coordination, formed by His66, His68, His112, and a water molecule, and a distorted trigonal bipyramidal environment, which additionally comprises Glu73. Manual docking of the substrate quercetin into the active site showed that the different geometries of the copper site might be of catalytic importance.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUH OCA].
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</div>
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<div class="pdbe-citations 1juh" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Crystal structure of the copper-containing quercetin 2,3-dioxygenase from Aspergillus japonicus., Fusetti F, Schroter KH, Steiner RA, van Noort PI, Pijning T, Rozeboom HJ, Kalk KH, Egmond MR, Dijkstra BW, Structure. 2002 Feb;10(2):259-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11839311 11839311]
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*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
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[[Category: Quercetin 2,3-dioxygenase]]
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== References ==
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[[Category: Dijkstra, B W.]]
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<references/>
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[[Category: Fusetti, F.]]
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__TOC__
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[[Category: Schroeter, K H.]]
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</StructureSection>
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[[Category: Steiner, R A.]]
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[[Category: Aspergillus japonicus]]
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[[Category: Beta sandwich]]
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[[Category: Large Structures]]
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[[Category: Copper]]
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[[Category: Dijkstra BW]]
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[[Category: Cupin]]
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[[Category: Fusetti F]]
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[[Category: Dioxygenase]]
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[[Category: Schroeter KH]]
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[[Category: Glycoprotein]]
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[[Category: Steiner RA]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:56:29 2008''
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Current revision

Crystal Structure of Quercetin 2,3-dioxygenase

PDB ID 1juh

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