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1juq

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[[Image:1juq.jpg|left|200px]]
 
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==GGA3 VHS domain complexed with C-terminal peptide from cation-dependent Mannose 6-phosphate receptor==
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The line below this paragraph, containing "STRUCTURE_1juq", creates the "Structure Box" on the page.
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<StructureSection load='1juq' size='340' side='right'caption='[[1juq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1juq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JUQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1juq| PDB=1juq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1juq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1juq OCA], [https://pdbe.org/1juq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1juq RCSB], [https://www.ebi.ac.uk/pdbsum/1juq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1juq ProSAT]</span></td></tr>
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</table>
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'''GGA3 VHS domain complexed with C-terminal peptide from cation-dependent Mannose 6-phosphate receptor'''
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== Function ==
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[https://www.uniprot.org/uniprot/MPRD_HUMAN MPRD_HUMAN] Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Specific sorting signals direct transmembrane proteins to the compartments of the endosomal-lysosomal system. Acidic-cluster-dileucine signals present within the cytoplasmic tails of sorting receptors, such as the cation-independent and cation-dependent mannose-6-phosphate receptors, are recognized by the GGA (Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding) proteins. The VHS (Vps27p, Hrs and STAM) domains of the GGA proteins are responsible for the highly specific recognition of these acidic-cluster-dileucine signals. Here we report the structures of the VHS domain of human GGA3 complexed with signals from both mannose-6-phosphate receptors. The signals bind in an extended conformation to helices 6 and 8 of the VHS domain. The structures highlight an Asp residue separated by two residues from a dileucine sequence as critical recognition elements. The side chains of the Asp-X-X-Leu-Leu sequence interact with subsites consisting of one electropositive and two shallow hydrophobic pockets, respectively. The rigid spatial alignment of the three binding subsites leads to high specificity.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/1juq_consurf.spt"</scriptWhenChecked>
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1JUQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUQ OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural basis for acidic-cluster-dileucine sorting-signal recognition by VHS domains., Misra S, Puertollano R, Kato Y, Bonifacino JS, Hurley JH, Nature. 2002 Feb 21;415(6874):933-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11859375 11859375]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1juq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Bonifacino, J S.]]
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[[Category: Bonifacino JS]]
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[[Category: Hurley, J H.]]
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[[Category: Hurley JH]]
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[[Category: Misra, S.]]
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[[Category: Misra S]]
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[[Category: Puertollano, R.]]
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[[Category: Puertollano R]]
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[[Category: Dxxll sorting signal]]
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[[Category: Protein-peptide compelx]]
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[[Category: Vhs domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:57:00 2008''
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Current revision

GGA3 VHS domain complexed with C-terminal peptide from cation-dependent Mannose 6-phosphate receptor

PDB ID 1juq

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