8fw3

From Proteopedia

(Difference between revisions)

Current revision

MtrR from Neisseria gonorrhoeae bound to Testosterone

Structural highlights

8fw3 is a 4 chain structure with sequence from Neisseria gonorrhoeae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.22Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MTRR_NEIGO Controls the permeability of the cell envelope to hydrophobic compounds such as antibiotics and detergents (PubMed:8196548). Represses transcription of the mtrCDE-encoded efflux pump by binding within the mtrCDE promoter (PubMed:9209024, PubMed:23221802). Also negatively regulates the expression of farR, by binding to its promoter region, leading indirectly to the positive regulation of expression of the farAB-encoded efflux pump (PubMed:14645274).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Transcriptional regulator MtrR inhibits the expression of the multidrug efflux pump operon mtrCDE in the pathogenic bacterium Neisseria gonorrhoeae. Here, we show that MtrR binds the hormonal steroids progesterone, beta-estradiol, and testosterone, which are present at urogenital infection sites, as well as ethinyl estrogen, a component of some hormonal contraceptives. Steroid binding leads to the decreased affinity of MtrR for cognate DNA, increased mtrCDE expression, and enhanced antimicrobial resistance. Furthermore, we solve crystal structures of MtrR bound to each steroid, thus revealing their binding mechanisms and the conformational changes that induce MtrR.

Hormonal steroids induce multidrug resistance and stress response genes in Neisseria gonorrhoeae by binding to MtrR.,Hooks GM, Ayala JC, Holley CL, Dhulipala V, Beggs GA, Perfect JR, Schumacher MA, Shafer WM, Brennan RG Nat Commun. 2024 Feb 7;15(1):1153. doi: 10.1038/s41467-024-45195-1. PMID:38326294^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee EH, Rouquette-Loughlin C, Folster JP, Shafer WM. FarR regulates the farAB-encoded efflux pump of Neisseria gonorrhoeae via an MtrR regulatory mechanism. J Bacteriol. 2003 Dec;185(24):7145-52. doi: 10.1128/jb.185.24.7145-7152.2003. PMID:14645274 doi:http://dx.doi.org/10.1128/jb.185.24.7145-7152.2003
↑ Zalucki YM, Dhulipala V, Shafer WM. Dueling regulatory properties of a transcriptional activator (MtrA) and repressor (MtrR) that control efflux pump gene expression in Neisseria gonorrhoeae. MBio. 2012 Dec 4;3(6):e00446-12. doi: 10.1128/mBio.00446-12. PMID:23221802 doi:http://dx.doi.org/10.1128/mBio.00446-12
↑ Pan W, Spratt BG. Regulation of the permeability of the gonococcal cell envelope by the mtr system. Mol Microbiol. 1994 Feb;11(4):769-75. doi: 10.1111/j.1365-2958.1994.tb00354.x. PMID:8196548 doi:http://dx.doi.org/10.1111/j.1365-2958.1994.tb00354.x
↑ Lucas CE, Balthazar JT, Hagman KE, Shafer WM. The MtrR repressor binds the DNA sequence between the mtrR and mtrC genes of Neisseria gonorrhoeae. J Bacteriol. 1997 Jul;179(13):4123-8. doi: 10.1128/jb.179.13.4123-4128.1997. PMID:9209024 doi:http://dx.doi.org/10.1128/jb.179.13.4123-4128.1997
↑ Hooks GM, Ayala JC, Holley CL, Dhulipala V, Beggs GA, Perfect JR, Schumacher MA, Shafer WM, Brennan RG. Hormonal steroids induce multidrug resistance and stress response genes in Neisseria gonorrhoeae by binding to MtrR. Nat Commun. 2024 Feb 7;15(1):1153. PMID:38326294 doi:10.1038/s41467-024-45195-1

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8fw3"

Categories: Large Structures | Neisseria gonorrhoeae | Brennan RG | Hooks GH

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8fw3 is ON HOLD  until Paper Publication
+==MtrR from Neisseria gonorrhoeae bound to Testosterone==
+<StructureSection load='8fw3' size='340' side='right'caption='[[8fw3]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8fw3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FW3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TES:TESTOSTERONE'>TES</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fw3 OCA], [https://pdbe.org/8fw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fw3 RCSB], [https://www.ebi.ac.uk/pdbsum/8fw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fw3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MTRR_NEIGO MTRR_NEIGO] Controls the permeability of the cell envelope to hydrophobic compounds such as antibiotics and detergents (PubMed:8196548). Represses transcription of the mtrCDE-encoded efflux pump by binding within the mtrCDE promoter (PubMed:9209024, PubMed:23221802). Also negatively regulates the expression of farR, by binding to its promoter region, leading indirectly to the positive regulation of expression of the farAB-encoded efflux pump (PubMed:14645274).<ref>PMID:14645274</ref> <ref>PMID:23221802</ref> <ref>PMID:8196548</ref> <ref>PMID:9209024</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Transcriptional regulator MtrR inhibits the expression of the multidrug efflux pump operon mtrCDE in the pathogenic bacterium Neisseria gonorrhoeae. Here, we show that MtrR binds the hormonal steroids progesterone, beta-estradiol, and testosterone, which are present at urogenital infection sites, as well as ethinyl estrogen, a component of some hormonal contraceptives. Steroid binding leads to the decreased affinity of MtrR for cognate DNA, increased mtrCDE expression, and enhanced antimicrobial resistance. Furthermore, we solve crystal structures of MtrR bound to each steroid, thus revealing their binding mechanisms and the conformational changes that induce MtrR.
-Authors: Hooks, G.H., Brennan, R.G.
+Hormonal steroids induce multidrug resistance and stress response genes in Neisseria gonorrhoeae by binding to MtrR.,Hooks GM, Ayala JC, Holley CL, Dhulipala V, Beggs GA, Perfect JR, Schumacher MA, Shafer WM, Brennan RG Nat Commun. 2024 Feb 7;15(1):1153. doi: 10.1038/s41467-024-45195-1. PMID:38326294<ref>PMID:38326294</ref>
-Description: MtrR from Neisseria gonorrhoeae bound to Testosterone
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Brennan, R.G]]
+<div class="pdbe-citations 8fw3" style="background-color:#fffaf0;"></div>
-[[Category: Hooks, G.H]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Neisseria gonorrhoeae]]
+[[Category: Brennan RG]]
+[[Category: Hooks GH]]

8fw3

From Proteopedia

Current revision

MtrR from Neisseria gonorrhoeae bound to Testosterone

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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