1jvt

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[[Image:1jvt.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF RIBONUCLEASE A (LIGAND-FREE FORM)==
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The line below this paragraph, containing "STRUCTURE_1jvt", creates the "Structure Box" on the page.
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<StructureSection load='1jvt' size='340' side='right'caption='[[1jvt]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jvt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JVT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jvt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jvt OCA], [https://pdbe.org/1jvt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jvt RCSB], [https://www.ebi.ac.uk/pdbsum/1jvt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jvt ProSAT]</span></td></tr>
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{{STRUCTURE_1jvt| PDB=1jvt | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jv/1jvt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jvt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Despite the increasing number of successful determinations of complex protein structures the understanding of their dynamics properties is still rather limited. Using X-ray crystallography, we demonstrate that ribonuclease A (RNase A) undergoes significant domain motions upon ligand binding. In particular, when cytidine 2'-monophosphate binds to RNase A, the structure of the enzyme becomes more compact. Interestingly, our data also show that these structural alterations are fully reversible in the crystal state. These findings provide structural bases for the dynamic behavior of RNase A in the binding of the substrate shown by Petsko and coworkers (Rasmussen et al. Nature 1992;357:423-424). These subtle domain motions may assume functional relevance for more complex system and may play a significant role in the cooperativity of oligomeric enzymes.
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'''CRYSTAL STRUCTURE OF RIBONUCLEASE A (LIGAND-FREE FORM)'''
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Reversible substrate-induced domain motions in ribonuclease A.,Vitagliano L, Merlino A, Zagari A, Mazzarella L Proteins. 2002 Jan 1;46(1):97-104. PMID:11746706<ref>PMID:11746706</ref>
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==Overview==
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Despite the increasing number of successful determinations of complex protein structures the understanding of their dynamics properties is still rather limited. Using X-ray crystallography, we demonstrate that ribonuclease A (RNase A) undergoes significant domain motions upon ligand binding. In particular, when cytidine 2'-monophosphate binds to RNase A, the structure of the enzyme becomes more compact. Interestingly, our data also show that these structural alterations are fully reversible in the crystal state. These findings provide structural bases for the dynamic behavior of RNase A in the binding of the substrate shown by Petsko and coworkers (Rasmussen et al. Nature 1992;357:423-424). These subtle domain motions may assume functional relevance for more complex system and may play a significant role in the cooperativity of oligomeric enzymes.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1JVT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVT OCA].
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</div>
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<div class="pdbe-citations 1jvt" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Reversible substrate-induced domain motions in ribonuclease A., Vitagliano L, Merlino A, Zagari A, Mazzarella L, Proteins. 2002 Jan 1;46(1):97-104. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11746706 11746706]
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Mazzarella L]]
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[[Category: Mazzarella, L.]]
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[[Category: Merlino A]]
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[[Category: Merlino, A.]]
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[[Category: Vitagliano L]]
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[[Category: Vitagliano, L.]]
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[[Category: Zagari A]]
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[[Category: Zagari, A.]]
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[[Category: Protein dynamic]]
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[[Category: Protein structure-function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:59:13 2008''
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CRYSTAL STRUCTURE OF RIBONUCLEASE A (LIGAND-FREE FORM)

PDB ID 1jvt

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