8i8c
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Plug structure of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)== | |
| + | <StructureSection load='8i8c' size='340' side='right'caption='[[8i8c]], [[Resolution|resolution]] 4.93Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8i8c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Autographa_californica_multiple_nucleopolyhedrovirus Autographa californica multiple nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I8C FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.93Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i8c OCA], [https://pdbe.org/8i8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i8c RCSB], [https://www.ebi.ac.uk/pdbsum/8i8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i8c ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0N7CQX9_9ABAC A0A0N7CQX9_9ABAC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) has been widely used as a bioinsecticide and a protein expression vector. Despite their importance, very little is known about the structure of most baculovirus proteins. Here, we show a 3.2 A resolution structure of helical cylindrical body of the AcMNPV nucleocapsid, composed of VP39, as well as 4.3 A resolution structures of both the head and the base of the nucleocapsid composed of over 100 protein subunits. AcMNPV VP39 demonstrates some features of the HK97-like fold and utilizes disulfide-bonds and a set of interactions at its C-termini to mediate nucleocapsid assembly and stability. At both ends of the nucleocapsid, the VP39 cylinder is constricted by an outer shell ring composed of proteins AC104, AC142 and AC109. AC101(BV/ODV-C42) and AC144(ODV-EC27) form a C14 symmetric inner layer at both capsid head and base. In the base, these proteins interact with a 7-fold symmetric capsid plug, while a portal-like structure is seen in the central portion of head. Additionally, we propose an application of AlphaFold2 for model building in intermediate resolution density. | ||
| - | + | Architecture of the baculovirus nucleocapsid revealed by cryo-EM.,Jia X, Gao Y, Huang Y, Sun L, Li S, Li H, Zhang X, Li Y, He J, Wu W, Venkannagari H, Yang K, Baker ML, Zhang Q Nat Commun. 2023 Nov 18;14(1):7481. doi: 10.1038/s41467-023-43284-1. PMID:37980340<ref>PMID:37980340</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Gao | + | <div class="pdbe-citations 8i8c" style="background-color:#fffaf0;"></div> |
| - | [[Category: Jia | + | == References == |
| - | [[Category: Zhang | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Autographa californica multiple nucleopolyhedrovirus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gao Y]] | ||
| + | [[Category: Jia X]] | ||
| + | [[Category: Zhang Q]] | ||
Current revision
Plug structure of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
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