1jwx

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[[Image:1jwx.jpg|left|200px]]
 
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==Chalcone Synthase--F215S mutant==
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The line below this paragraph, containing "STRUCTURE_1jwx", creates the "Structure Box" on the page.
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<StructureSection load='1jwx' size='340' side='right'caption='[[1jwx]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jwx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
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{{STRUCTURE_1jwx| PDB=1jwx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwx OCA], [https://pdbe.org/1jwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwx RCSB], [https://www.ebi.ac.uk/pdbsum/1jwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwx ConSurf].
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<div style="clear:both"></div>
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'''Chalcone Synthase--F215S mutant'''
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==See Also==
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*[[Chalcone synthase|Chalcone synthase]]
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== References ==
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==Overview==
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<references/>
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Type III polyketide synthases (PKS) generate an array of natural products by condensing multiple acetyl units derived from malonyl-CoA to thioester-linked starter molecules covalently bound in the PKS active site. One strategy adopted by Nature for increasing the functional diversity of these biosynthetic enzymes involves modifying polyketide assembly by altering the preference for starter molecules. Chalcone synthase (CHS) is a ubiquitous plant PKS and the first type III PKS described functionally and structurally. Guided by the three-dimensional structure of CHS, Phe-215 and Phe-265, which are situated at the active site entrance, were targeted for site-directed mutagenesis to diversify CHS activity. The resulting mutants were screened against a panel of aliphatic and aromatic CoA-linked starter molecules to evaluate the degree of starter molecule specificity in CHS. Although wild-type CHS accepts a number of natural CoA thioesters, it does not use N-methylanthraniloyl-CoA as a substrate. Substitution of Phe-215 by serine yields a CHS mutant that preferentially accepts this CoA-thioester substrate to generate a novel alkaloid, namely N-methylanthraniloyltriacetic acid lactone. These results demonstrate that a point mutation in CHS dramatically shifts the molecular selectivity of this enzyme. This structure-based approach to metabolic redesign represents an initial step toward tailoring the biosynthetic activity of plant type III PKS.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1JWX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWX OCA].
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==Reference==
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Expanding the biosynthetic repertoire of plant type III polyketide synthases by altering starter molecule specificity., Jez JM, Bowman ME, Noel JP, Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5319-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11959984 11959984]
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[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
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[[Category: Naringenin-chalcone synthase]]
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[[Category: Bowman ME]]
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[[Category: Single protein]]
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[[Category: Jez JM]]
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[[Category: Bowman, M E.]]
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[[Category: Noel JP]]
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[[Category: Jez, J M.]]
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[[Category: Noel, J P.]]
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[[Category: Altered substrate specificity]]
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[[Category: Ketoacyl synthase]]
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[[Category: Polyketide synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:01:41 2008''
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Current revision

Chalcone Synthase--F215S mutant

PDB ID 1jwx

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