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2lsv

From Proteopedia

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Current revision

The NMR high resolution structure of yeast Tah1 in complex with the Hsp90 C-terminal tail

Structural highlights

2lsv is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TAH1_YEAST

Publication Abstract from PubMed

The ubiquitous Hsp90 chaperone participates in snoRNP and RNA polymerase assembly through interaction with the R2TP complex. This complex includes the proteins Tah1, Pih1, Rvb1, and Rvb2. Tah1 bridges Hsp90 to R2TP. Its minimal TPR domain includes two TPR motifs and a capping helix. We established the high-resolution solution structures of Tah1 free and in complex with the Hsp90 C-terminal peptide. The TPR fold is similar in the free and bound forms and we show experimentally that in addition to its solvating/stabilizing role, the capping helix is essential for the recognition of the Hsp90 704EMEEVD709 motif. In addition to Lys79 and Arg83 from the carboxylate clamp, this helix bears Tyr82 forming a pi/S-CH3 interaction with Hsp90 M705 from the peptide 310 helix. The Tah1 C-terminal region is unfolded, and we demonstrate that it is essential for the recruitment of the Pih1 C-terminal domain and folds upon binding.

High-Resolution Structural Analysis Shows How Tah1 Tethers Hsp90 to the R2TP Complex.,Back R, Dominguez C, Rothe B, Bobo C, Beaufils C, Morera S, Meyer P, Charpentier B, Branlant C, Allain FH, Manival X Structure. 2013 Sep 4. pii: S0969-2126(13)00296-7. doi:, 10.1016/j.str.2013.07.024. PMID:24012479^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Back R, Dominguez C, Rothe B, Bobo C, Beaufils C, Morera S, Meyer P, Charpentier B, Branlant C, Allain FH, Manival X. High-Resolution Structural Analysis Shows How Tah1 Tethers Hsp90 to the R2TP Complex. Structure. 2013 Sep 4. pii: S0969-2126(13)00296-7. doi:, 10.1016/j.str.2013.07.024. PMID:24012479 doi:10.1016/j.str.2013.07.024

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lsv"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2lsv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LSV FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lsv OCA], [https://pdbe.org/2lsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lsv RCSB], [https://www.ebi.ac.uk/pdbsum/2lsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lsv ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lsv OCA], [https://pdbe.org/2lsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lsv RCSB], [https://www.ebi.ac.uk/pdbsum/2lsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lsv ProSAT]</span></td></tr>
 </table>
 == Function ==

2lsv

From Proteopedia

Current revision

The NMR high resolution structure of yeast Tah1 in complex with the Hsp90 C-terminal tail

Structural highlights

Function

Publication Abstract from PubMed

References

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