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2m3s

From Proteopedia

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Current revision

Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant

Structural highlights

2m3s is a 1 chain structure with sequence from Gallus gallus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CALM_CHICK Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases.

Publication Abstract from PubMed

It only takes one mutation: A strategically placed single mutation in a non-enzymatic protein scaffold produced AlleyCat, a small, allosterically regulated catalyst of Kemp elimination. In only seven rounds of directed evolution the enzymatic efficiency of the original 74 amino acid residue catalyst was improved more than 220-fold to achieve a kcat value higher than that of catalytic antibodies for the same reaction, still preserving allosteric regulation.

A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction.,Moroz OV, Moroz YS, Wu Y, Olsen AB, Cheng H, Mack KL, McLaughlin JM, Raymond EA, Zhezherya K, Roder H, Korendovych IV Angew Chem Int Ed Engl. 2013 Jun 10;52(24):6246-9. doi: 10.1002/anie.201302339., Epub 2013 Apr 29. PMID:23630096^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Moroz OV, Moroz YS, Wu Y, Olsen AB, Cheng H, Mack KL, McLaughlin JM, Raymond EA, Zhezherya K, Roder H, Korendovych IV. A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction. Angew Chem Int Ed Engl. 2013 Jun 10;52(24):6246-9. doi: 10.1002/anie.201302339., Epub 2013 Apr 29. PMID:23630096 doi:10.1002/anie.201302339

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2m3s"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2m3s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M3S FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m3s OCA], [https://pdbe.org/2m3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m3s RCSB], [https://www.ebi.ac.uk/pdbsum/2m3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m3s ProSAT]</span></td></tr>
 </table>

2m3s

From Proteopedia

Current revision

Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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