1k1q

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[[Image:1k1q.gif|left|200px]]
 
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==Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus==
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The line below this paragraph, containing "STRUCTURE_1k1q", creates the "Structure Box" on the page.
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<StructureSection load='1k1q' size='340' side='right'caption='[[1k1q]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1k1q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K1Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k1q OCA], [https://pdbe.org/1k1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k1q RCSB], [https://www.ebi.ac.uk/pdbsum/1k1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k1q ProSAT]</span></td></tr>
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{{STRUCTURE_1k1q| PDB=1k1q | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus'''
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[https://www.uniprot.org/uniprot/DPO4_SACSO DPO4_SACSO] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k1/1k1q_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1K1Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1Q OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k1q ConSurf].
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Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus., Silvian LF, Toth EA, Pham P, Goodman MF, Ellenberger T, Nat Struct Biol. 2001 Nov;8(11):984-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11685247 11685247]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Sulfolobus solfataricus]]
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</StructureSection>
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[[Category: Ellenberger, T.]]
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[[Category: Large Structures]]
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[[Category: Goodman, M F.]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Pham, P.]]
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[[Category: Ellenberger T]]
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[[Category: Silvian, L F.]]
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[[Category: Goodman MF]]
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[[Category: Toth, E A.]]
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[[Category: Pham P]]
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[[Category: Dna polymerase]]
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[[Category: Silvian LF]]
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[[Category: Error-prone polymerase]]
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[[Category: Toth EA]]
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[[Category: Lesion-bypass polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:11:30 2008''
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Current revision

Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus

PDB ID 1k1q

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